<![CDATA[Sterlization grade filtering?]]>Having been on the bad end of a Reta batch that ended up failing sterility tests, I started down the rabbit hole of filters. I have been filtering using "sterile" 22 micron filters since I started researching peptides to help my wombat, but on researching what to do about this most recent test failure I realized that all filters are not made equally.

I found that the pass/fail that janoshik gives for sterilization tests is not enough information to just move on, filter, and use failed peptides (not that I would, I'm just researching facts) and that the amount of "stuff" that a filter can clean up is limited - the filter can be overwhelmed, so you need need to know the level of failure for any sterility test if you were to theoretically want to remediate the contamination.

Also I now understand that "normal" 22u filters are not up to the standard of "sterilization grade" filters and are perhaps not enough to give me the safety I'm looking for. The ones I found most commonly listed are the PureFlo D65 Disc Filter 0.65um / 0.2um - Sterile Compounding (Aqueous), but I'm having trouble finding a source these high grade filters.

With all that said, what does Randy think about better filters and would he know a source for the Pureflo D65 filters?

]]>https://community.peptidecritic.com/topic/1171/sterlization-grade-filteringRSS for NodeThu, 23 Apr 2026 18:00:13 GMTTue, 21 Apr 2026 14:47:25 GMT60<![CDATA[Reply to Sterlization grade filtering? on Thu, 23 Apr 2026 14:42:38 GMT]]>Also...Sorry guys, I left off the decimal in the filters i was describing. Meant to say ".22 micron" or ".22um". I guess nobody noticed, but 22um and .22um are vastly different things lol.

]]>https://community.peptidecritic.com/post/4808https://community.peptidecritic.com/post/4808Thu, 23 Apr 2026 14:42:38 GMT<![CDATA[Reply to Sterlization grade filtering? on Thu, 23 Apr 2026 12:39:43 GMT]]>After more reading this week I honestly believe the only difference between the "sterilization grade" filters and the sterile ones we are already using is the certification tests and related marking. Those tests are just spot checks though so I would think batches would still vary. Bottom line, it's all a calculated risk. Do the things you can do to make the calculations more likely to come out in your favor based on your comfort level.

]]>https://community.peptidecritic.com/post/4799https://community.peptidecritic.com/post/4799Thu, 23 Apr 2026 12:39:43 GMT<![CDATA[Reply to Sterlization grade filtering? on Wed, 22 Apr 2026 20:08:19 GMT]]>Love it!

]]>https://community.peptidecritic.com/post/4724https://community.peptidecritic.com/post/4724Wed, 22 Apr 2026 20:08:19 GMT<![CDATA[Reply to Sterlization grade filtering? on Wed, 22 Apr 2026 19:56:00 GMT]]>said in Sterlization grade filtering?:

Worthy of a look and priced attractively: Cobetter 0.2μm Hydrophilic PVDF Syringe Filters for Sterile Filtration

Those who care can investigate PES vs PVDF (both great) for water based agents.

cobetter1.jpg co2.jpg

Gratuitous supplies pics...lol 😋

]]>https://community.peptidecritic.com/post/4722https://community.peptidecritic.com/post/4722Wed, 22 Apr 2026 19:56:00 GMT<![CDATA[Reply to Sterlization grade filtering? on Wed, 22 Apr 2026 19:45:46 GMT]]>Worthy of a look and priced attractively: Cobetter 0.2μm Hydrophilic PVDF Syringe Filters for Sterile Filtration

]]>https://community.peptidecritic.com/post/4721https://community.peptidecritic.com/post/4721Wed, 22 Apr 2026 19:45:46 GMT<![CDATA[Reply to Sterlization grade filtering? on Wed, 22 Apr 2026 17:42:04 GMT]]>@kj4otu .22 micron pes filters do the trick.

]]>https://community.peptidecritic.com/post/4712https://community.peptidecritic.com/post/4712Wed, 22 Apr 2026 17:42:04 GMT<![CDATA[Reply to Sterlization grade filtering? on Wed, 22 Apr 2026 14:21:43 GMT]]>I guess I've stumped everybody? LOL @randy any thoughts on the need for sterilization grade filtering?

]]>https://community.peptidecritic.com/post/4698https://community.peptidecritic.com/post/4698Wed, 22 Apr 2026 14:21:43 GMT