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Freedom Again

Scheduled Pinned Locked Moved Peptide Discussion
55 Posts 14 Posters 1.2k Views 1 Watching
Freedom Again Freedom Again
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  • pep_researcherP pep_researcher

    @ogsteve This is not right. Endotoxins residues even without active bacteria can cause serious adverse research outcomes and they don't get filtered out using regular pep filters.

    Recall that JEEP Tirz batches with horrendous endotoxins levels had passed sterility tests. You can find COAs on this forum.

    O Offline
    O Offline
    ogsteve
    wrote last edited by ogsteve
    #44

    @pep_researcher maybe re-read my comment. I never said anything about filtering endotoxins. 😉

    Endo bad…
    Sterilty… dont care. Just filter.

    The Endo that’s in the vial doesn’t get any worse while it’s in the vial.

    The bacteria that cause the endo get filtered at recon.

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    • O ogsteve

      @pep_researcher maybe re-read my comment. I never said anything about filtering endotoxins. 😉

      Endo bad…
      Sterilty… dont care. Just filter.

      The Endo that’s in the vial doesn’t get any worse while it’s in the vial.

      The bacteria that cause the endo get filtered at recon.

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      Stevepep
      wrote last edited by Stevepep
      #45

      @ogsteve the basic fact you are missing is if there is a sterility issue there are endotoxins they go hand and hand and most labs suck at testing for them where sterility is fairly easy to test for.

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      • S Stevepep

        @ogsteve the basic fact you are missing is if there is a sterility issue there are endotoxins they go hand and hand and most labs suck at testing for them where sterility is fairly easy to test for.

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        ogsteve
        wrote last edited by ogsteve
        #46

        @Stevepep i didnt miss that. Im just able to address them separately and still succeed. ✊

        One needs $150 testing to find in one vial out of a batch

        One needs $0.65 to SOLVE every single vial.

        If endo is over spec its garbage. If its not sterile but under endo then filter solves the remaining issue.

        This is what i mean when i say i dont care much if they test for sterilty. Its a “nice to have”.

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        • O ogsteve

          @Stevepep i didnt miss that. Im just able to address them separately and still succeed. ✊

          One needs $150 testing to find in one vial out of a batch

          One needs $0.65 to SOLVE every single vial.

          If endo is over spec its garbage. If its not sterile but under endo then filter solves the remaining issue.

          This is what i mean when i say i dont care much if they test for sterilty. Its a “nice to have”.

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          mikebricker
          wrote last edited by
          #47

          @ogsteve which filters do you use? 0.22 or 0.10? Thanks.

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          • M mikebricker

            @ogsteve which filters do you use? 0.22 or 0.10? Thanks.

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            ogsteve
            wrote last edited by ogsteve
            #48

            @mikebricker Yes. (Thanks to your guidance i might add)

            Depends on the pep and how sketchy it is.

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            • O ogsteve

              @mikebricker Yes. (Thanks to your guidance i might add)

              Depends on the pep and how sketchy it is.

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              mikebricker
              wrote last edited by
              #49

              @ogsteve how do you determine sketchiness?

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              • M mikebricker

                @ogsteve how do you determine sketchiness?

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                Stevepep
                wrote last edited by
                #50

                @mikebricker Better question why would you take a sketchy peptide? This crap catches up to you and when it does people change their opinions pretty quick. Not talking about you. You aren't solving everything with a filter, most bacteria is large enough but there are smaller pathogens than can get you. Its just an extra safety measure that one should absolutely use IMO

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                • M mikebricker

                  @ogsteve how do you determine sketchiness?

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                  ogsteve
                  wrote last edited by
                  #51

                  @mikebricker well if its from china directly and has
                  Minimal Testing then it gets the .10. If its from mile
                  High maybe the .22. Most commercial products like glutathione or cerebrolysin or cortexin fall into this category.

                  Alternatively something like ara-290 that wants sterile water or pbs recon its definitely super sterile procedures with masks and 0.10 and temp controlled storage, expirations etc.

                  BullseyeB 1 Reply Last reply
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                  • O ogsteve

                    @Stevepep i didnt miss that. Im just able to address them separately and still succeed. ✊

                    One needs $150 testing to find in one vial out of a batch

                    One needs $0.65 to SOLVE every single vial.

                    If endo is over spec its garbage. If its not sterile but under endo then filter solves the remaining issue.

                    This is what i mean when i say i dont care much if they test for sterilty. Its a “nice to have”.

                    S Online
                    S Online
                    Stevepep
                    wrote last edited by
                    #52

                    @ogsteve said:

                    @Stevepep i didnt miss that. Im just able to address them separately and still succeed. ✊

                    One needs $150 testing to find in one vial out of a batch

                    One needs $0.65 to SOLVE every single vial.

                    If endo is over spec it’s garbage. If itnot sterile but inder endo then filter solves the remaining issue.

                    Generally speaking I agree. The issue is many labs have been proven to be extremely inaccurate on endotoxins because it requires expensive equipment , a clean room trained technicians etc etc. that’s why many don’t report a value and just say pass/fail because they filter out a set amount knowing the environment isn’t clean enough to accurately test. Others report a value with a filtered number built in. Sterility you can generally rely on. It’s an easy test. If it fails sterility it has endotoxins maybe not at a toxic level per se but you should stay away from a product that fails filter or not. If the environment was that unclean there also could be other contaminants like viruses that a filter will not filter out.

                    I just hope nobody is seeing these posts and think they can take a peptide that failed sterility and just filter it and be ok. That’s is asking for trouble

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                    • BullseyeB Offline
                      BullseyeB Offline
                      Bullseye
                      wrote last edited by
                      #53

                      Freedom has turned out to be just like AFI. We wonder if they even test the stuff. They are garbage.

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                      • O ogsteve

                        @mikebricker well if its from china directly and has
                        Minimal Testing then it gets the .10. If its from mile
                        High maybe the .22. Most commercial products like glutathione or cerebrolysin or cortexin fall into this category.

                        Alternatively something like ara-290 that wants sterile water or pbs recon its definitely super sterile procedures with masks and 0.10 and temp controlled storage, expirations etc.

                        BullseyeB Offline
                        BullseyeB Offline
                        Bullseye
                        wrote last edited by
                        #54

                        @ogsteve Hi Steve. Where are you finding the .10 filters. I have the Cobetter .22's. Would like to add a few .1's for those ones with minimal testing.

                        Thank you.

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                        • BullseyeB Bullseye

                          @ogsteve Hi Steve. Where are you finding the .10 filters. I have the Cobetter .22's. Would like to add a few .1's for those ones with minimal testing.

                          Thank you.

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                          Stevepep
                          wrote last edited by Stevepep
                          #55

                          @Bullseye keep in mind a .1 will not filter endotoxins they are microscopic. It will filter additional bacteria like mycoplasma that lack a cell wall. If you have those in the vial you have other things to worry about and you won’t get them all. You will know when you get Pneumonia though. No reason.not to do it other than they clog far easier and pressure can affect the stability of certain peptides. I have tried them several times. Go very slow is my advice. If you do a search people have linked suppliers they aren’t cheap.

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