Does filtering work? Yep, lab tested....
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@diannemccann Yes specifically these are designed for sterility and endotoxins and are the best way for a single vial peptide purchaser to deal with S&E if all they have is IPN on a peptide. Cytiva (PALL) 4908-N Acrodisc Syringe Filters Optimized for Scale Up, Posidyne membrane, sterile, 0.2 µm 25mm. .22 is the standard not .1
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I wanted to share this in the hopes that others might start sharing more testing info here on Peptide Critic....
I kept seeing people recommending to filter their peps and started doing some research. I found that the .2 micron filters, while good, aren't 100% for bacteria. Granted the bacteria they would miss are very rare and would not be commonly found in lyophilized peptides, but that less than 100% still bothered me.
Considering there has to be a practical and financial limit to the rabbit hole we go down for research peptide safety, and we all have to find our own comfort level with that, I started doing some more research.
I found sterile 0.1 micron filters for about $1 each and decided to go one step further and actually test them. We had a batch of Reta from JEEP that failed sterility at Janoshik. (also note that this is when we found out that Janoshik will allow someone to edit a report to change the company name, batch number, etc after the fact for a small fee. Def a red flag for doctoring "verified" tests at Janoshik).
I sent in two of those vials and two of the 0.1 Micron filters that I found. I had them reconstitute the two contaminated vials and test half of each vial unfiltered and half of each vial filtered. This confirmed for me that filtering (at least with the 0.1 micron filters) is viable and effective. Note I mistyped the MG size as 24mg instead of 34mg doh. Hope this is useful for somebody.
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Nice specific testing. Thanks!
I guess what we don't know with these results is wether the peptide itself survived the 0.1 micron filtration or not.
Btw, I tested the exact same batch, included endotoxins. These came good.
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@kRazy-pep Syringe filters cannot remove endotoxins. Endotoxins require special ultra resolution filter which may filter out entire peptide content leaving it useless for research. Only other way to remove endotoxins is high temp autoclaving. Not sure once endotoxins get into pep API whether they can be removed. The only way is to AVOID letting them in.
Syringe filters can only remove microbial contents and other larger impurities.
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@kRazy-pep No, the batch that failed endo was Tirz, this is Reta.
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@kRazy-pep Syringe filters cannot remove endotoxins. Endotoxins require special ultra resolution filter which may filter out entire peptide content leaving it useless for research. Only other way to remove endotoxins is high temp autoclaving. Not sure once endotoxins get into pep API whether they can be removed. The only way is to AVOID letting them in.
Syringe filters can only remove microbial contents and other larger impurities.
@pep_researcher Yes, I seem to be mixing up sterility and endo... I keep cracking my head on the sides of the rabbit hole as I tumble down.
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Me, being a Medical Technologist, would be interested to know what bacteria it grew.
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I have some reta to would that destroy it with the filter being so tiny? If no, would it remove endo? #oldguy
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@flyhigh endotoxins are cell walls these filters are not going to be successful filtering out tiny molecules like that. Bacteria and other contaminants yes but endotoxins are too small
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I just placed an order here, not sure how long it will take yet. I’ll keep you updated. But they have free shipping. I’ve ordered from them before, not medical related and they shipped quickly.
I just placed an order here, not sure how long it will take yet. I’ll keep you updated. But they have free shipping. I’ve ordered from them before, not medical related and they shipped quickly.
Did the same, never shipped.
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After doing a bit of research it appears that the biggest concern when using the .1 um 13mm filter is pressure. The filter will not block the compounds and they will easily pass through but to much pressure when pushing on the plunger could damage the the structure of the the larger compounds like Reta, TB500(tb4). As long as you don’t push to quickly you should be fine. About 20-30 seconds to filter 2ml is a good safe pace.
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Where do you find empty sterile vials to put the filtered peptide into? I can’t find any at a decent price and the ones on Amazon have reviews that show vials that are obviously not sterile. Thanks!
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Where do you find empty sterile vials to put the filtered peptide into? I can’t find any at a decent price and the ones on Amazon have reviews that show vials that are obviously not sterile. Thanks!
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Thanks for the responses, I appreciate it!
What are pen carts, and are they already sterile? Does anyone have a link to amazon or somewhere to see how to order them? When I look up pen carts it shows me actual rolling carts lol Thanks!!!@alleal Cartridges. They're pretty standard - 3ml, and they work with all the pens. They are here in the store, and feel a bit more trustworthy for sterility, but you can get them on Amazon or anywhere. Look under tutorials for how to filter directly into the cartridge once you've reconstituted.
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Thanks for the responses, I appreciate it!
What are pen carts, and are they already sterile? Does anyone have a link to amazon or somewhere to see how to order them? When I look up pen carts it shows me actual rolling carts lol Thanks!!!
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