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Peptide Critic Community Peptide Critic Community
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  3. Does filtering work? Yep, lab tested....
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Does filtering work? Yep, lab tested....

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Does filtering work? Yep, lab tested.... Does filtering work? Yep, lab tested....
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  • K kRazy-pep

    @tincho This is the same batch R30 that failed the endo test a bit ago?

    pep_researcherP Online
    pep_researcherP Online
    pep_researcher
    wrote last edited by pep_researcher
    #53

    @kRazy-pep Syringe filters cannot remove endotoxins. Endotoxins require special ultra resolution filter which may filter out entire peptide content leaving it useless for research. Only other way to remove endotoxins is high temp autoclaving. Not sure once endotoxins get into pep API whether they can be removed. The only way is to AVOID letting them in.

    Syringe filters can only remove microbial contents and other larger impurities.

    K 1 Reply Last reply
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    • K kRazy-pep

      @tincho This is the same batch R30 that failed the endo test a bit ago?

      T Offline
      T Offline
      tincho
      wrote last edited by
      #54

      @kRazy-pep No, the batch that failed endo was Tirz, this is Reta.

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      • pep_researcherP pep_researcher

        @kRazy-pep Syringe filters cannot remove endotoxins. Endotoxins require special ultra resolution filter which may filter out entire peptide content leaving it useless for research. Only other way to remove endotoxins is high temp autoclaving. Not sure once endotoxins get into pep API whether they can be removed. The only way is to AVOID letting them in.

        Syringe filters can only remove microbial contents and other larger impurities.

        K Offline
        K Offline
        kRazy-pep
        wrote last edited by
        #55

        @pep_researcher Yes, I seem to be mixing up sterility and endo... I keep cracking my head on the sides of the rabbit hole as I tumble down.

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        • S Stevepep

          @OldGuy wow great work and terrific information

          C Offline
          C Offline
          cecilwebbjr
          wrote last edited by
          #56

          @Stevepep said:

          @OldGuy wow great work and terrific information

          Me, being a Medical Technologist, would be interested in knowing what bacteria it grew.

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          • C Offline
            C Offline
            cecilwebbjr
            wrote last edited by
            #57

            Me, being a Medical Technologist, would be interested to know what bacteria it grew.

            1 Reply Last reply
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            • R remwar

              I have some reta to would that destroy it with the filter being so tiny? If no, would it remove endo? #oldguy

              F Offline
              F Offline
              flyhigh
              wrote last edited by
              #58

              @remwar probably

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              • S Stevepep

                @flyhigh endotoxins are cell walls these filters are not going to be successful filtering out tiny molecules like that. Bacteria and other contaminants yes but endotoxins are too small

                F Offline
                F Offline
                flyhigh
                wrote last edited by
                #59
                This post is deleted!
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                • Snake PepskinS Snake Pepskin

                  I just placed an order here, not sure how long it will take yet. I’ll keep you updated. But they have free shipping. I’ve ordered from them before, not medical related and they shipped quickly.

                  https://www.zoro.com/globe-scientific-syringe-filter-13-mm-dia-10-ml-pk50-sf-pes-1013-s/i/G903673742/

                  T Offline
                  T Offline
                  tincho
                  wrote last edited by
                  #60

                  @Snake-Pepskin said:

                  I just placed an order here, not sure how long it will take yet. I’ll keep you updated. But they have free shipping. I’ve ordered from them before, not medical related and they shipped quickly.

                  https://www.zoro.com/globe-scientific-syringe-filter-13-mm-dia-10-ml-pk50-sf-pes-1013-s/i/G903673742/

                  Did the same, never shipped.

                  T 1 Reply Last reply
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                  • J Offline
                    J Offline
                    jveitenhans
                    wrote last edited by
                    #61

                    After doing a bit of research it appears that the biggest concern when using the .1 um 13mm filter is pressure. The filter will not block the compounds and they will easily pass through but to much pressure when pushing on the plunger could damage the the structure of the the larger compounds like Reta, TB500(tb4). As long as you don’t push to quickly you should be fine. About 20-30 seconds to filter 2ml is a good safe pace.

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                    • A Offline
                      A Offline
                      alleal
                      wrote last edited by
                      #62

                      Where do you find empty sterile vials to put the filtered peptide into? I can’t find any at a decent price and the ones on Amazon have reviews that show vials that are obviously not sterile. Thanks!

                      RandyR M 2 Replies Last reply
                      0
                      • A alleal

                        Where do you find empty sterile vials to put the filtered peptide into? I can’t find any at a decent price and the ones on Amazon have reviews that show vials that are obviously not sterile. Thanks!

                        RandyR Offline
                        RandyR Offline
                        Randy
                        wrote last edited by
                        #63

                        @alleal just filter them into the pen carts.

                        "If it doesnt come in a needle. It doesn't work"

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                        • A alleal

                          Where do you find empty sterile vials to put the filtered peptide into? I can’t find any at a decent price and the ones on Amazon have reviews that show vials that are obviously not sterile. Thanks!

                          M Offline
                          M Offline
                          mary
                          wrote last edited by
                          #64

                          @alleal

                          @Randy said:

                          @alleal just filter them into the pen carts.

                          This! And he has a video on Rumble for filtering demo.

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                          • A Offline
                            A Offline
                            alleal
                            wrote last edited by alleal
                            #65

                            Thanks for the responses, I appreciate it!
                            What are pen carts, and are they already sterile? Does anyone have a link to amazon or somewhere to see how to order them? When I look up pen carts it shows me actual rolling carts lol Thanks!!!

                            S M 2 Replies Last reply
                            0
                            • A alleal

                              Thanks for the responses, I appreciate it!
                              What are pen carts, and are they already sterile? Does anyone have a link to amazon or somewhere to see how to order them? When I look up pen carts it shows me actual rolling carts lol Thanks!!!

                              S Offline
                              S Offline
                              SkepticJen
                              wrote last edited by
                              #66

                              @alleal Cartridges. They're pretty standard - 3ml, and they work with all the pens. They are here in the store, and feel a bit more trustworthy for sterility, but you can get them on Amazon or anywhere. Look under tutorials for how to filter directly into the cartridge once you've reconstituted.

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                              • A alleal

                                Thanks for the responses, I appreciate it!
                                What are pen carts, and are they already sterile? Does anyone have a link to amazon or somewhere to see how to order them? When I look up pen carts it shows me actual rolling carts lol Thanks!!!

                                M Offline
                                M Offline
                                mary
                                wrote last edited by
                                #67

                                @alleal

                                "pen cartridge for peptides"

                                https://www.amazon.com/s?k=pen+cartridge+for+peptides&crid=QD1SJAXKC9W2&sprefix=pen+cartr%2Caps%2C211&ref=nb_sb_ss_p13n-expert-pd-ops-ranker_2_9

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                                • A Offline
                                  A Offline
                                  alleal
                                  wrote last edited by alleal
                                  #68

                                  Once I filter my peptides do I still need to check for sterility when I get them tested at janoshik? It’s an extra $290 just for the sterility test and the cost is braking the bank:)
                                  Thanks for all the information, you are all amazing willing to take the time to educate someone!

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                                  • ResearchCatR Offline
                                    ResearchCatR Offline
                                    ResearchCat
                                    wrote last edited by
                                    #69
                                    This post is deleted!
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                                    • T tincho

                                      @Snake-Pepskin said:

                                      I just placed an order here, not sure how long it will take yet. I’ll keep you updated. But they have free shipping. I’ve ordered from them before, not medical related and they shipped quickly.

                                      https://www.zoro.com/globe-scientific-syringe-filter-13-mm-dia-10-ml-pk50-sf-pes-1013-s/i/G903673742/

                                      Did the same, never shipped.

                                      T Offline
                                      T Offline
                                      tincho
                                      wrote last edited by
                                      #70

                                      tincho said:

                                      @Snake-Pepskin said:

                                      I just placed an order here, not sure how long it will take yet. I’ll keep you updated. But they have free shipping. I’ve ordered from them before, not medical related and they shipped quickly.

                                      https://www.zoro.com/globe-scientific-syringe-filter-13-mm-dia-10-ml-pk50-sf-pes-1013-s/i/G903673742/

                                      Did the same, never shipped.

                                      Update: They finally shipped. Filtering goes very smooth thru these. I think it worth it for some extra filtration.

                                      M 1 Reply Last reply
                                      0
                                      • T tincho

                                        tincho said:

                                        @Snake-Pepskin said:

                                        I just placed an order here, not sure how long it will take yet. I’ll keep you updated. But they have free shipping. I’ve ordered from them before, not medical related and they shipped quickly.

                                        https://www.zoro.com/globe-scientific-syringe-filter-13-mm-dia-10-ml-pk50-sf-pes-1013-s/i/G903673742/

                                        Did the same, never shipped.

                                        Update: They finally shipped. Filtering goes very smooth thru these. I think it worth it for some extra filtration.

                                        M Online
                                        M Online
                                        mikebricker
                                        wrote last edited by
                                        #71
                                        This post is deleted!
                                        M 1 Reply Last reply
                                        0
                                        • M mikebricker

                                          This post is deleted!

                                          M Online
                                          M Online
                                          mikebricker
                                          wrote last edited by
                                          #72

                                          Do they come individually wrapped?

                                          1 Reply Last reply
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