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Equipment for doing your own testing

Scheduled Pinned Locked Moved Supplies, Mixing & Storage
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  • RandyR Offline
    RandyR Offline
    Randy
    wrote last edited by Randy
    #8

    6b1d8722-b992-460a-8463-c67435c79466-image.jpeg

    Just saying...theyve got a good margin on that.

    Talk to them like a grey market vendor and say "Hello Dear, I need sample before i make big order and do much business" Then the MOQ doesnt matter.

    "If it doesnt come in a needle. It doesn't work"

    Reactive3078R D 2 Replies Last reply
    4
    • RandyR Offline
      RandyR Offline
      Randy
      wrote last edited by
      #9

      I also dont know what this machine is actually capable of. I have asked someone much smarter who runs a lab.

      "If it doesnt come in a needle. It doesn't work"

      1 Reply Last reply
      0
      • RandyR Randy

        6b1d8722-b992-460a-8463-c67435c79466-image.jpeg

        Just saying...theyve got a good margin on that.

        Talk to them like a grey market vendor and say "Hello Dear, I need sample before i make big order and do much business" Then the MOQ doesnt matter.

        Reactive3078R Offline
        Reactive3078R Offline
        Reactive3078
        wrote last edited by
        #10

        @Randy said:

        "Hello Dear, I need sample before i make big order and do much business"

        😂😂😂

        D 1 Reply Last reply
        0
        • C Commander

          That link is not for any test equipment.... it is a peptide vendor

          D Offline
          D Offline
          djoelcassell
          wrote last edited by
          #11

          @Commander 51f36b1e-2785-4f11-a5a3-dc62efbbdcd2-image.jpeg

          Sorry about that. The link was with me logged in. Yes, it's AMC peptide vendor but I was not suggesting people buy peptides from there. I have in the past but not in the last year or more. Just happened to see recently that they sell equipment too. I found it interesting.

          Again, apologies for not thinking the link through.

          Joel

          1 Reply Last reply
          0
          • Reactive3078R Reactive3078

            @Randy said:

            "Hello Dear, I need sample before i make big order and do much business"

            😂😂😂

            D Offline
            D Offline
            djoelcassell
            wrote last edited by
            #12

            @Reactive3078 - Bahahahha! Again, I'm not recommending the machine or AMCs peptides, I just didn't know that testing equipment that SUPPOSEDLY does the same tests we pay for, was attainable for us common folks. I own a winery and I KNOW how expensive equipment is. I assumed specialized peptide testing equipment would run, like winery equipment, $100-$250K minimum. I do have a small wine analyzer that tests for five or six things but even it runs $25K.

            Randy you can take this down anytime brother, I'm not affiliated in any way other than I made 3-4 peptide purchases from AMC in 2024-25. Ha! It may be a piece of junk. Just thought it was interesting.

            Joel

            1 Reply Last reply
            0
            • RandyR Randy

              6b1d8722-b992-460a-8463-c67435c79466-image.jpeg

              Just saying...theyve got a good margin on that.

              Talk to them like a grey market vendor and say "Hello Dear, I need sample before i make big order and do much business" Then the MOQ doesnt matter.

              D Offline
              D Offline
              djoelcassell
              wrote last edited by djoelcassell
              #13

              @Randy Oh my! That's quite a margin! And even more attainable than I thought! Hahaha. Now I feel like an idiot.

              And in light of the Snake Pepskins quote under his name: “Knowledge is the ultimate power.
              It’s not what you know that defines you, it’s that which you share, that defines you.”

              I REALLY feel like an idiot for sharing that link, Lol!

              RandyR 1 Reply Last reply
              0
              • S Offline
                S Offline
                SSB
                wrote last edited by
                #14

                People mix up three different questions:

                Purity - what fraction of the UV-absorbing stuff is the main peak
                Identity - is that peak the peptide on the label
                Amount - how many mg of actual peptide are in the vial

                A used Agilent 1100/1200 + C18 column + 0.1 mg balance will do #1 and a version of #3. It will not do #2. FOr #2 you need a Mass Spec machine.
                HPLC separates by hydrophobicity and measures peak area at 214/220 nm. Purity is just:
                main peak area / all peak areas

                Practical cheap setup for HPLC: complete used 1100/1200 gradient HPLC with UV/DAD, one new C18, HPLC ACN/water/TFA, 0.1 mg balance. Ballpark $8–15k could be less on used lab equipment sites but need to make sure it has all the modules. Not easy to keep these machines working well without knowhow.

                For identity of what is in the vial you need Mass Spec
                Best cheap option: ESI single-quad LC-MS

                Agilent 6120/6130/6140, Shimadzu LCMS-2020, Waters QDa/SQD.

                Used working ones usually ask $35k–$55k. “Powers on” eBay units at $8k–$20k are often dead. Also really tough to run and maintain - need nitrogen tank at home etc.

                D 1 Reply Last reply
                1
                • S SSB

                  People mix up three different questions:

                  Purity - what fraction of the UV-absorbing stuff is the main peak
                  Identity - is that peak the peptide on the label
                  Amount - how many mg of actual peptide are in the vial

                  A used Agilent 1100/1200 + C18 column + 0.1 mg balance will do #1 and a version of #3. It will not do #2. FOr #2 you need a Mass Spec machine.
                  HPLC separates by hydrophobicity and measures peak area at 214/220 nm. Purity is just:
                  main peak area / all peak areas

                  Practical cheap setup for HPLC: complete used 1100/1200 gradient HPLC with UV/DAD, one new C18, HPLC ACN/water/TFA, 0.1 mg balance. Ballpark $8–15k could be less on used lab equipment sites but need to make sure it has all the modules. Not easy to keep these machines working well without knowhow.

                  For identity of what is in the vial you need Mass Spec
                  Best cheap option: ESI single-quad LC-MS

                  Agilent 6120/6130/6140, Shimadzu LCMS-2020, Waters QDa/SQD.

                  Used working ones usually ask $35k–$55k. “Powers on” eBay units at $8k–$20k are often dead. Also really tough to run and maintain - need nitrogen tank at home etc.

                  D Offline
                  D Offline
                  djoelcassell
                  wrote last edited by
                  #15

                  @BS That's good to know, and tracks with good winery equipment as I was thinking just a little lower priced. Fortunately I have Nitrogen and Argon gas here all the time for filling headspace in my barrels and tanks, but even so, not pulling the trigger on Mass Spec machine. Know winery equipment maintenance but not this type of equipment. Not maintaining it properly is probably the same though, untrustworthy outputs and failing equipment.

                  Thanks for your knowledge!

                  1 Reply Last reply
                  1
                  • D djoelcassell

                    @Randy Oh my! That's quite a margin! And even more attainable than I thought! Hahaha. Now I feel like an idiot.

                    And in light of the Snake Pepskins quote under his name: “Knowledge is the ultimate power.
                    It’s not what you know that defines you, it’s that which you share, that defines you.”

                    I REALLY feel like an idiot for sharing that link, Lol!

                    RandyR Offline
                    RandyR Offline
                    Randy
                    wrote last edited by
                    #16

                    @djoelcassell you are good. I had no idea this product existed so you sent me down the rabbit hole 😉.

                    "If it doesnt come in a needle. It doesn't work"

                    C 1 Reply Last reply
                    0
                    • RandyR Randy

                      @djoelcassell you are good. I had no idea this product existed so you sent me down the rabbit hole 😉.

                      C Offline
                      C Offline
                      Commander
                      wrote last edited by
                      #17

                      @Randy
                      Did you buy it yet…

                      Please excuse my typos. Small Phone & Bad Eyes

                      1 Reply Last reply
                      0
                      • RandyR Offline
                        RandyR Offline
                        Randy
                        wrote last edited by
                        #18

                        nah. it wont do what we want.

                        "If it doesnt come in a needle. It doesn't work"

                        1 Reply Last reply
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