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Biuret for Concentrations Comparison

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  • mmpM Offline
    mmpM Offline
    mmp
    wrote last edited by mmp
    #1

    Has anyone compared the same peptide concentrations using the Biuret reaction?
    I mean comparing the colors of the mixture in 0.5 ml syringes—something like this:
    0.1 mL sample1 + 0.1 mL Biuret
    0.1 mL sample2 + 0.1 mL Biuret

    AI said that this way I can see if the difference exceeds 15%.

    Can be useful for cases like that
    https://substack.com/@krysia0430/note/c-378942145

    S 1 Reply Last reply
    0
    • mmpM mmp

      Has anyone compared the same peptide concentrations using the Biuret reaction?
      I mean comparing the colors of the mixture in 0.5 ml syringes—something like this:
      0.1 mL sample1 + 0.1 mL Biuret
      0.1 mL sample2 + 0.1 mL Biuret

      AI said that this way I can see if the difference exceeds 15%.

      Can be useful for cases like that
      https://substack.com/@krysia0430/note/c-378942145

      S Offline
      S Offline
      Stevepep
      wrote last edited by
      #2

      @mmp I don't follow I use it on all peptides other than Nexaph when I reconstitute. The color purple intensity will vary based on the number of peptide chains. For instance, KPV will be light purple. Tesa would be a much deeper purple.

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      • S Offline
        S Offline
        Stevepep
        wrote last edited by
        #3

        Let me know what you are trying to do happy to help

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