Lab vs Lab, We tested 5 Labs using the same Kit.
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This is a great article, thanks for doing the research, it is truly helpful!!!
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New Multi‑Lab COA Deep Dive: Why Test Results Vary Between Labs
We just published a new breakdown on a multi‑lab retatrutide test that should be interesting for anyone who actually reads COAs instead of just the bold numbers.
In this run, the same "Uncle Wang" Retatrutide 10 mg kit from Nexaph was tested by six different labs: AFI, Freedom Diagnostics, Janoshik (vendor and third‑party), TrustPointe Analytics, and Vanguard Laboratory. The labs all agreed on the big picture: identity confirmed as retatrutide and very high purity (roughly 99.7–99.9%+ depending on the report).
Where things got spicy was the mass / net content. Reported content ranged from about 10.18 mg up to 12.80 mg per vial, with some labs clustering low (AFI, Vanguard) and others higher (Freedom, TrustPointe, Janoshik). Instead of treating that spread as “who’s lying,” we walk through why those numbers can legitimately differ across methods, calibration curves, sample prep, vial selection, and how each lab defines and calculates content.
https://peptidecritic.com/blog/why-test-results-vary-between-labs-multi-lab-coa-comparison
@Randy Being in this space has taught me so much about “all things researching peptides “. I am grateful for the Youtube videos also which brought me here. I had to look in my freezer after reading all the COA’s on the Reta. Coincidentally, I just purchased that same green crimp ring with the transparent purple cap of a 10mg kit of Reta from Nexaph. Oh yes, and found Nexaph on this space too. Thanks Jeff/Randy
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@hectoraponte Unc Wang only does limited runs of 10 bottles at a time...or i bought a sticker maker.
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@Randy where can i get “wangs peptides” for randys nephew?
@hectoraponte said in Lab vs Lab, We tested 5 Labs using the same Kit.:
@Randy where can i get “wangs peptides” for randys nephew?
Same!!!
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@hectoraponte Unc Wang only does limited runs of 10 bottles at a time...or i bought a sticker maker.
Thank you randy!
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@beachfun We have a second kit of this we are using for some other degridation tests. Lio'd on a windowsill, in the fridge and in the glove box of my truck. Check back in 3 months.
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I just watched a video that said the true way to test if a product is what it actually is sold as is to have it tested with Liquid Chromatography-Mass Spectrometer (LC-MS) to actually tell if it actually the correct peptide. I thought that is what a purity test covered, but looking at this image above, I guess I am wrong.
What vendors are tested like that and what testing labs are actually using this? I see Freedom might be, if I am reading that right.
I apologies in advance, new to COA process and trying to learn more on it.
video that I randomly came across:
https://youtu.be/lHUOLJHiJzA?si=9P0QJVayqogka9fc&t=488@rogerp thanks for posting this -- did you find an LC-MS lab?
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@rogerp thanks for posting this -- did you find an LC-MS lab?
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@randy Do you think kits are ever mixed?
I have the mind set that it’s just business and people in china are doing the right thing… I still pin it anyway@jimmy4407th what do you mean, mixed?
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@jimmy4407th what do you mean, mixed?
@Randy I should have clarified. Different patches in one kit.
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So how do you determine your mass to use if it varies from different vendor?
I recently had GHK-CU 50mg order. The vendor COA (Jano) had two vials. 54.84 and 55.22. The second party vendor paid COA (Jano) came back at 54.83, 52.31, and 53.24. Close to the first but just a slightly lower average. The 3rd party COA (Trustpointe) came back a fair amount lower at 51.280 and 50.735. Are you still taking an average of all vials to use as your vial's mass for reconstituion? -
So how do you determine your mass to use if it varies from different vendor?
I recently had GHK-CU 50mg order. The vendor COA (Jano) had two vials. 54.84 and 55.22. The second party vendor paid COA (Jano) came back at 54.83, 52.31, and 53.24. Close to the first but just a slightly lower average. The 3rd party COA (Trustpointe) came back a fair amount lower at 51.280 and 50.735. Are you still taking an average of all vials to use as your vial's mass for reconstituion?@Nuncles
This is exactly why I do not use the “test” among for my calculations when reconing it.
Had an entire kit tested using multiple labs and they all varied. Some came in at 18.xx and others came in at 20.xx.. I think one might have even came back 22.xx, can’t remember exactly.
I just use the label amount (int my case 20m)) unless it comes back a lot lower, like the SS-31 from a group buy that came back at 40mg. In that case, I use the 40mg -
@Nuncles
This is exactly why I do not use the “test” among for my calculations when reconing it.
Had an entire kit tested using multiple labs and they all varied. Some came in at 18.xx and others came in at 20.xx.. I think one might have even came back 22.xx, can’t remember exactly.
I just use the label amount (int my case 20m)) unless it comes back a lot lower, like the SS-31 from a group buy that came back at 40mg. In that case, I use the 40mg@Commander That's exactly what I thinking in the beginning. I was going to use the "label" amount. Since we never know exactly how much is in any individual vial, it will never be exact. I had just figured the dose would be a little strong or a little weak depending on the actual content in a particular vial. But the more I kept reading, the more I saw people stating they use the average since it may make a big difference in some peptides. But the average varying so much in different tests throws a big wrench in the theory of using the average as a standard.
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@Commander That's exactly what I thinking in the beginning. I was going to use the "label" amount. Since we never know exactly how much is in any individual vial, it will never be exact. I had just figured the dose would be a little strong or a little weak depending on the actual content in a particular vial. But the more I kept reading, the more I saw people stating they use the average since it may make a big difference in some peptides. But the average varying so much in different tests throws a big wrench in the theory of using the average as a standard.
@Nuncles
Exactly. I bought 20 Kits of Reta 20mg. 15 are for me and 5 for a friend. The 4 or 5 labs came back with different values. So how can you possibly use the average? I also noticed a trend with the labs. Most of the time they seem to be consistent with the difference between them.
So what labs results do you use to average them for dose? Unless you have a true manufacturer that intentionally overfills (I believe Lobster does this), you really can’t average them unless they are way off. I had MOTS-C that tested 49mg (labeled 40mg), I might do it with that big of a variation, but not with a 20mg that tests at 21.xx by one lab because it could test different by another.
Some labs seem to be off more times than not.
And what if you are averaging 22.3, 22.6, & 22.1 for reta 20mg because say iLS came up with those numbers. Then you have a problem and you “feel” like it isn’t really as strong as the Reta you got from a different vendor before. But your previous vendors test results tested at 18.59 & 18.78 and you averaged those numbers. And you “felt” like the Vendors Reta was better or “stronger” it simply could be that it really was 20mg but the way that lab tests come up with lower numbers.
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