Glutathione (GSH) Subcutaneous Research Dosing Protocol

[Randy]

For research use only. Not medical advice.

Overview

Glutathione (GSH) is the body’s primary intracellular antioxidant, widely researched for oxidative-stress reduction, detoxification pathways, and cellular protection.
Subcutaneous administration is commonly used in moderate, recurring weekly doses.

Weekly Dosing Protocol (Subcutaneous)

Standard Weekly Range

100–200 mg per injection

2–3× weekly
Typical weekly total: 300–600 mg

This provides sustained antioxidant support without oversaturating metabolic pathways.

Titration Schedule

Weeks 1–2 — Initiation Phase

100 mg per injection

2–3× weekly
Useful for assessing tolerance (rarely an issue with GSH).

Weeks 3–8 — Maintenance Phase

300 mg per injection

3× weekly
This is the most common long-term research dosing pattern.

Weeks 9–12 — Extended Phase

200 mg per injection

3× weekly
Continuation for prolonged oxidative-stress or detoxification-focused models.

Frequency Options

Common research schedules:

3× Weekly (Most Popular)

Monday / Wednesday / Friday

Produces steady-state systemic antioxidant activity

2× Weekly

Used when a milder protocol is desired

Typically 200 mg twice weekly

Daily Micro-Dosing (Optional)

50–100 mg daily

Used in some dermatologic or oxidative-stress research models

Not required for standard systemic antioxidant protocols

Cycle Length

Typical GSH research cycles:

6–12 weeks, followed by

2–4 weeks off, if desired

Some researchers continue long-term without breaks due to excellent tolerability.

Common Research Notes

Subcutaneous GSH is generally well tolerated; mild warmth at the injection site is the most common reaction.

GSH stacks well with NAC, vitamin C, ALA, and mitochondrial-support protocols.

Effects tend to be subtle but cumulative — best evaluated over several weeks.

If used alongside peptides that increase oxidative load, GSH may reduce recovery time and improve subjective well-being.

[diegoc]

I did my first Glutathione shot today 100mg with Taurine in the Bac water…

Felt no pain…

I will do 3x a week 100mg first week and up the dose to 200mg which is 100units 1ml shot

[Randy]

have a source for the the glutathione transport bac?

[diegoc]

This is the one i ordered…

https://nexusaminos.com/products/gtb-20ml

[reinventing_hell]

I have Fuan 600mg gluta vials, do you think 3ml BAC is enough for it?

[Randy]

The good stuff!

only one way to find out...

I wouldnt suggest it. the lowest ive gone is 5ml and that made my left love handle a no go zone for a week.

[reinventing_hell]

how much BAC do you usually use and for what concentration? First time researching Gluta.

[Randy]

i did 7ml the first time.... Tried 5. now i use 9

[PeptidePete]

I ordered some of the 2400mg Glutathione from Nexus Aminos along with their Glutathione Transport Bac Water. The Glut vials are TWENTY ML!

I'm not going to get away with a 5ml dilution of that am I?

Intention is to do 200-400mg pins. Greater dilution=2x,3x,4x pins per day to hit the desired dose. Decisions decisions...

[PeptideEd]

When I looked into this... I found references to standard concentration for glutathione injections being ~200 mg/ml. When I ordered a 1500 mg bottle from Eternal Peptides (which the COA had at about 1650 mg) I chose to use 9 ml to reconstitute it. From a sting on injection point of view worked well for me. That said... I only used normal BAC water... very interested in trying the taurine enhanced bac water next time.

[jeremy2026]

@reinventing_hell said in Glutathione (GSH) Subcutaneous Research Dosing Protocol:

I have Fuan 600mg gluta vials, do you think 3ml BAC is enough for it?

Hey @reinventing_hell, where did you buy Fuan gluta?

[jeremy2026]

@PeptidePete said in Glutathione (GSH) Subcutaneous Research Dosing Protocol:

Glutathione from Nexus Aminos

@peptidepete Does the Glutathione from Nexus Aminos come with a batch number on the vial so it can be linked to the COA?

[PeptidePete]

@jeremy2026 said in Glutathione (GSH) Subcutaneous Research Dosing Protocol:

@PeptidePete said in Glutathione (GSH) Subcutaneous Research Dosing Protocol:

Glutathione from Nexus Aminos

@peptidepete Does the Glutathione from Nexus Aminos come with a batch number on the vial so it can be linked to the COA?

Great question that I had not even considered. I will post an answer in four days when it arrives

[diegoc]

Im the one that posted the Bac water with Taurine from them but i only bought the water not the Glutathione

[diegoc]

@randy hey bud, have you ever researched with mixing the Bac , Taurine water with any other peptides such as GHK-Cu of Mots-C

[Randy]

i have not. Im one of the lucky ones where 10iu per mg of ghk doesnt bother me. any less and its questionable.

[diegoc]

@Randy so far my limit is 2.5mg with around 40-50u of extra bac water.

I preload them in a luer lock and add a 33g 8mm and works for me,

Do you think Taurine Bac will comprise GHK, i think its worth a test

[PeptidePete]

said in Glutathione (GSH) Subcutaneous Research Dosing Protocol:

@jeremy2026 said in Glutathione (GSH) Subcutaneous Research Dosing Protocol:

@PeptidePete said in Glutathione (GSH) Subcutaneous Research Dosing Protocol:

Glutathione from Nexus Aminos

@peptidepete Does the Glutathione from Nexus Aminos come with a batch number on the vial so it can be linked to the COA?

Great question that I had not even considered. I will post an answer in four days when it arrives

It does NOT come with any identifying numbers or codes, at all

[Randy]

@diegoc ill ask the lab guy but my guess is probably not.

[Randy]

@PeptidePete photos of the bottle? cap etc?