Testing Sterility of Reconstituted/Used Vials with Sterile Water
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Hey all,
I’ve been really interested in the differences between bacteriostatic water and sterile water use for reconstitution. I understand the general consensus is that bacteriostatic water is the best and safest bet and the majority of researchers will only use this for their studies. As I’m new to all of this I will probably do the same as well.
But I was really intrigued by what Janoshik had to say in his interview regarding no access to bac water in EU and sterile water being primary source/used for 28 days refrigerated. I know there are opinions on him lol but it intrigued me nonetheless.
My rat has relatively easy access to Hospira sterile water for injection and am wondering if it’s worth to reconstitute with and draw from once a week for a month and test whether or not anything will grow from it. It’s likely i could get a 5 day growth plate done but I’ll ask my rat’s lab friend to see if longer tests are possible.
I’m also curious of the results if I transfer that 28 day sample to a sterile vial for testing and have the original vial tested after 90 days continuing the same protocol once a week.
Obviously this has no say in the degradation of the peptide but at least what I’ve read in the forums, sterility is the largest concern for continued use?
Anybody have anything to add regarding how I should go about this? Should I try and see if I could also have another vial just sitting at room temp for the 28 day duration but still being drawn from once a week? Is it even worth it to reconstitute and just continue to draw from a regular SWI vial? I haven’t consulted my rat’s lab friend about it but after a recent conversation with the lab using the extra space in the incubator for testing items for personal intrigue they mentioned being willing to test anything.
I would appreciate anybody also with any knowledge in this field to add. I think it’s interesting how the word sterile is used as well in this field. I’m used to the term being very strict and everything having to be in a closed environment (under a hood, sterile gloves, masks being used, no hands “leaving” sterile field, etc etc). In placing intravenous access for patients that we use to draw blood from and push medication/fluids in, we typically don’t do it in a sterile fashion. Wiping with alcohol is not considered sterile, it’s considered clean. Any other HCP that also research I would love to chat about this. Not hating on anyone by the way! I understand I’m a noob in this field. Just appreciate anyone with any insight. Keeping an open mind
