Lab vs Lab, We tested 5 Labs using the same Kit.
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New Multi‑Lab COA Deep Dive: Why Test Results Vary Between Labs

We just published a new breakdown on a multi‑lab retatrutide test that should be interesting for anyone who actually reads COAs instead of just the bold numbers.
In this run, the same "Uncle Wang" Retatrutide 10 mg kit from Nexaph was tested by six different labs: AFI, Freedom Diagnostics, Janoshik (vendor and third‑party), TrustPointe Analytics, and Vanguard Laboratory. The labs all agreed on the big picture: identity confirmed as retatrutide and very high purity (roughly 99.7–99.9%+ depending on the report).
Where things got spicy was the mass / net content. Reported content ranged from about 10.18 mg up to 12.80 mg per vial, with some labs clustering low (AFI, Vanguard) and others higher (Freedom, TrustPointe, Janoshik). Instead of treating that spread as “who’s lying,” we walk through why those numbers can legitimately differ across methods, calibration curves, sample prep, vial selection, and how each lab defines and calculates content.
https://peptidecritic.com/blog/why-test-results-vary-between-labs-multi-lab-coa-comparison
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Fantastic article!! See, this is why I love this space. Just gets down to the brass tacks with a no, nonsense approach. I learn so much from this platform everyday.
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Awesome work & sorta what I thought would be found.
One question - were the labs sent more than 1X vial aware they were all from the same source/you?
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Awesome work & sorta what I thought would be found.
One question - were the labs sent more than 1X vial aware they were all from the same source/you?
@STATIEEIGHT 2 vials from the kit sent to each lab, plus the Jank result from 3rd party test on thr same batch and the vendors 2nd party test from jano on the same batxh.
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I ain't upset that Nexaph overfills its vials a tad
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As I've come to expect from @randy, science is explained in simple, understandable terms that provide real-world applicability while calling out the "Stone Tablet" argument of COA's from any particular lab. I followed this original discussion with great interest and am delighted to see this write-up. Thanks for the time, money, and effort you spent on this.
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Awesome, thank you very much for the research!
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Awesome work & sorta what I thought would be found.
One question - were the labs sent more than 1X vial aware they were all from the same source/you?
@STATIEEIGHT John at AFI is a friend (we still pay him). The rest of them probably saw peptide critic on the credit card if they were looking for it.
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Love the info and REALLY love those labels on the vials, hilarious and so freaking creative. Thank you for this!
@JustAgirl I bought a sticker maker thinking id do something funny...i never thought it would involve unc wang.
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I just watched a video that said the true way to test if a product is what it actually is sold as is to have it tested with Liquid Chromatography-Mass Spectrometer (LC-MS) to actually tell if it actually the correct peptide. I thought that is what a purity test covered, but looking at this image above, I guess I am wrong.
What vendors are tested like that and what testing labs are actually using this? I see Freedom might be, if I am reading that right.
I apologies in advance, new to COA process and trying to learn more on it.
video that I randomly came across:
https://youtu.be/lHUOLJHiJzA?si=9P0QJVayqogka9fc&t=488 -
I don't see reta on their website, will they be selling or sold reta? New to this world, and this is seems to be a signal of who to trust in a world that seems really hard to find a trustworthy provider.
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I don't see reta on their website, will they be selling or sold reta? New to this world, and this is seems to be a signal of who to trust in a world that seems really hard to find a trustworthy provider.
@islander671 nxp-3p

