3 ml syringe

[Eleanor]

@kristal FYI, I just reconstituted some Cartalax using breather and it's too small. Stick with a bit bigger needle. Filter had a hard time with it and I was real careful but not worth the effort or chance of breaking it.

[kristal]

@Eleanor thank you - 27 might be a good size.

[Eleanor]

@kristal Yep, forget the breather, trust me.

[mrjoshua44]

These are what I use for reconstitution and breather needles, a 25Ga-1 1/2in needle and a 30Ga-5/16in.

https://a.co/d/07FtDw6L

https://a.co/d/01S4JWgn

[ResearchCat]

I like the 31 ga 1-1/2” version for loading pens. It lets me get every drop out of the vial and is a tiny puncture in the cart.

Yes, it is a very slow draw so you have to be patient.

[Newenough]

This post is deleted!

[matthew]

Hi,
just used a 3ml syringe for first time when drawing bac water/reconstituting and when i inserted into the vial the plunger dropped expelling the bac into the vial at a way faster rate than i usually reconstitute.
A small amount of bubble appeared but dispersed almost immediately.
Sorry for the stupid question but is that why breathers are used, to reduce vacuum pressure? and would the rapid rate/bubbles affect the quality of my research results?

[MyB]

@matthew the breather is for filling pen vials.
People who claim to be experts will tell you introduce bac slowly and run it down the side of the vial. Peptides aren’t as fragile as some people say. Your research material is most likely fine.

[Eleanor]

@matthew Surprised me the first time too; has quite a pull to it. Even though some people don't mind the fast fill, I hold that plunger somewhat in place and slow it down. If you didn't insert a breather needle to the side of where you're placing your 3ml syringe needle, give that a try; it won't stop the plunger from dispensing but makes it easier.

[matthew]

thanks for the info and tips
cheers