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Peptide Critic Community

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  1. Randy the Rats Research Forum
  2. Peptide Discussion
  3. Testing for Endotoxins.
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Testing for Endotoxins.

Scheduled Pinned Locked Moved Peptide Discussion
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  • M Offline
    M Offline
    mikebricker
    wrote last edited by
    #1

    I’ve been looking at this web site for a few months now, and really like it.
    It seems that the top referenced sellers like EX, Mile High, etc all seem to get good, >99% purity.

    But, some of even the highest rated do not report Endotoxin results. For your own information, <5EU/mg is NOT a numbered result. If you look at a few sellers, they actually report the result like 0.078EU/mg for example.
    Reporting <5EU/mg means it could be 4.9.

    A report listing "< 5 EU/mg" usually means the laboratory set their testing equipment to a highly lenient detection threshold. Rather than looking for absolute trace purity, they are only confirming that the sample is free of massive, systemic bacterial contamination.

    A < 5 EU/mg specification is the absolute baseline bare-minimum for chemical legitimacy. Premium vendors looking to eliminate variables will typically test past this threshold to prove their batches achieve the much stricter limits—like < 0.05 EU/mL or even better an actual number if not <0.05.

    So look at the COA’s presented from your favorite sellers. If the actual Endo isn’t reported, my recommendation is to move on to a cleaner set of samples from a different seller.

    S 1 Reply Last reply
    0
    • M mikebricker

      I’ve been looking at this web site for a few months now, and really like it.
      It seems that the top referenced sellers like EX, Mile High, etc all seem to get good, >99% purity.

      But, some of even the highest rated do not report Endotoxin results. For your own information, <5EU/mg is NOT a numbered result. If you look at a few sellers, they actually report the result like 0.078EU/mg for example.
      Reporting <5EU/mg means it could be 4.9.

      A report listing "< 5 EU/mg" usually means the laboratory set their testing equipment to a highly lenient detection threshold. Rather than looking for absolute trace purity, they are only confirming that the sample is free of massive, systemic bacterial contamination.

      A < 5 EU/mg specification is the absolute baseline bare-minimum for chemical legitimacy. Premium vendors looking to eliminate variables will typically test past this threshold to prove their batches achieve the much stricter limits—like < 0.05 EU/mL or even better an actual number if not <0.05.

      So look at the COA’s presented from your favorite sellers. If the actual Endo isn’t reported, my recommendation is to move on to a cleaner set of samples from a different seller.

      S Online
      S Online
      Stevepep
      wrote last edited by Stevepep
      #2

      @mikebricker IMO endotoxin testing at US labs is a joke. Most, if not all, are false positives. You are better off relying on Sterility. They don't have the environmental controls in place to properly test, so they end up introducing them into the sample. There are endotoxins everywhere, on every surface. If you look at videos of these labs they shouldn't even consider endotoxin testing. I won't even get into the equipment and training requirements.

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      • NewenoughN Offline
        NewenoughN Offline
        Newenough
        wrote last edited by
        #3

        @stevepep I think the one exception is Jeep. I'm pretty sure they add extra Endo's. 😂

        S vpeptidesV 2 Replies Last reply
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        • NewenoughN Newenough

          @stevepep I think the one exception is Jeep. I'm pretty sure they add extra Endo's. 😂

          S Online
          S Online
          Stevepep
          wrote last edited by
          #4

          @Newenough that’s hysterical. That was a Janoshik test and at least they try to have the right environmental conditions to test for it.

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          • NewenoughN Newenough

            @stevepep I think the one exception is Jeep. I'm pretty sure they add extra Endo's. 😂

            vpeptidesV Offline
            vpeptidesV Offline
            vpeptides
            wrote last edited by
            #5

            @Newenough Endotoxins are very good for your immunity, didn't you know?

            attentiion is all you need

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            • N Online
              N Online
              Nuncles
              wrote last edited by
              #6

              Wouldn't this be more of a lab issue than vendor?

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              • RandyR Randy moved this topic from Grey Zone

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