Nexaph testing
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AI suggests that the small 2% peak appearing slightly earlier than the main peak is most likely an oxidized variant of the peptide (specifically at the Met27 position), or less commonly, a synthesis deletion sequence that left the chain slightly more polar. Neither of these indicates external contaminants; they are simply minor structural variations of the Tesamorelin molecule itself.
Exactly. Broken chains of proteins can affect the purity, big-time. At first glance looking at something that says 97% , one might assume there's a sterility/contaminent issue, but often it's not. Think of it more as a potency issue.
This doesn't mean it gets a pass btw. At $288 for a kit, for that price I would expect 99%
@Neil-McCauley I wouldn't buy it, but think about how many peptides are bought with 97pct or less purity that show a COA of 99 plus from other retailers that use low quality labs. Nexaph has the perfect example of that: on their last batch of Tesa, they could have just used the Freedom 99.7pct results and not shown the Jano 97pct results, and a lot of people would have bought it hook, line, and sinker, as they do from other retailers they purchase from.
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@PeptideEd thats shitty. I will say tesa is one of the harder ones to deal with on their end. Unacceptable either way.
Maybe its time to hit up our girl Tracy at QSC and get on the real big boy juice
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@Neil-McCauley I wouldn't buy it, but think about how many peptides are bought with 97pct or less purity that show a COA of 99 plus from other retailers that use low quality labs. Nexaph has the perfect example of that: on their last batch of Tesa, they could have just used the Freedom 99.7pct results and not shown the Jano 97pct results, and a lot of people would have bought it hook, line, and sinker, as they do from other retailers they purchase from.
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I wouldn't sweat over 97% knowing it is nothing else but an oxidized or a broken molecule of the same, not any contaminant. The more important to know is that it is the real tesamorelin, it binds to the proper receptors, it is folded correctly, the amino acids are chained in a proper order. No 99.9% COA from Janoshik can guarantee it, it can well be a completely differently connected chain but have the same HPLC and MS results. So, the most important parameter is how trustworthy is the manufacturer.
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@Jdot I have asked and the response is along the lines of Tesa is tough not what I was hoping for. At least. we have an honest COA and can make an informed choice. I need Tesa soon so this kind of sucks. I just posted in their telegram as well on this
@Jdot I have asked and the response is along the lines of Tesa is tough not what I was hoping for. At least. we have an honest COA and can make an informed choice. I need Tesa soon so this kind of sucks. I just posted in their telegram as well on this
This actually concerns me now knowing that Tesa is "tough" as literally every other vendor out there always has 99.xx%+ from labs like ILS, FREEDOM, KOVERA, etc. So either those labs aren't testing correctly and Jano is now, or something is going on with Nexaph. I think Nexaph is top notch also and an honest company, so I'm thinking the other labs are not testing correctly and thats why its hard to find Tesa below 99% anywhere from all these resellers.
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@Jdot I have asked and the response is along the lines of Tesa is tough not what I was hoping for. At least. we have an honest COA and can make an informed choice. I need Tesa soon so this kind of sucks. I just posted in their telegram as well on this
This actually concerns me now knowing that Tesa is "tough" as literally every other vendor out there always has 99.xx%+ from labs like ILS, FREEDOM, KOVERA, etc. So either those labs aren't testing correctly and Jano is now, or something is going on with Nexaph. I think Nexaph is top notch also and an honest company, so I'm thinking the other labs are not testing correctly and thats why its hard to find Tesa below 99% anywhere from all these resellers.
@denti it’s appears it has to do with updated control samples. Janoshik updates their control samples which essentially are 100pct purity. They are always improving testing and some labs don’t even use a control sample hmm wonder which ones. I don’t completely get this explanation does that mean it was really always 97pct in other batches but the control samples weren’t up to the standard. I am kind of lost on this one.
Bottom line you buy from another source who uses a lab you can’t rely on that says 99pct or buy from Nexaph and it’s 97pct. We already have seen the last batch freedom COA. If it makes us feel better we can just forget the Janoshik one.
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@denti it’s appears it has to do with updated control samples. Janoshik updates their control samples which essentially are 100pct purity. They are always improving testing and some labs don’t even use a control sample hmm wonder which ones. I don’t completely get this explanation does that mean it was really always 97pct in other batches but the control samples weren’t up to the standard. I am kind of lost on this one.
Bottom line you buy from another source who uses a lab you can’t rely on that says 99pct or buy from Nexaph and it’s 97pct. We already have seen the last batch freedom COA. If it makes us feel better we can just forget the Janoshik one.
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@Stevepep that explanation is weird, how updating the control sample explains that extra bump on Mass Spectroscopy chart? It is a peak of a different mass. The new sample could affect ID test, HPLC, not a purity test, to my understanding.
@bobs outside my area of expertise I would think a 100 percent pure standard used to calibrate equipment would have a big impact on purity levels I would think that’s part of the purpose. The equipment is calibrated to the pure peptide. The blip I have no clue what that is I am going to just copy it and ask Janoshik I wouldn’t 100 pct trust AI on this stuff. We are finding AI to be wrong very often in my company
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@bobs outside my area of expertise I would think a 100 percent pure standard used to calibrate equipment would have a big impact on purity levels I would think that’s part of the purpose. The equipment is calibrated to the pure peptide. The blip I have no clue what that is I am going to just copy it and ask Janoshik I wouldn’t 100 pct trust AI on this stuff. We are finding AI to be wrong very often in my company
@Stevepep this is not my expertise either, I am an engineer and a "citizen scientist". But it is a general knowledge how to read these distribution charts, integrating AUC at different masses. I guess, if you test a 100% pure compound, you should see one narrow peak. Having two peaks side by side should indicate molecules of two different masses at some concentration. I am puzzled to see how calibration may affect it. A less sensitive equipment may place both peaks too close together though to see them separately. Again, just my speculations here.
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@Stevepep this is not my expertise either, I am an engineer and a "citizen scientist". But it is a general knowledge how to read these distribution charts, integrating AUC at different masses. I guess, if you test a 100% pure compound, you should see one narrow peak. Having two peaks side by side should indicate molecules of two different masses at some concentration. I am puzzled to see how calibration may affect it. A less sensitive equipment may place both peaks too close together though to see them separately. Again, just my speculations here.
@bobs my understanding is the equipment is calibrated at many labs to just look for the peptide without any reference it’s a catalogue standard. The reference standard allows the equipment to fine tune for the peptide and only the peptide so without the reference that other blip that is close to the long arrow would be just filtered and included as part of the peptide. It was described as shooting a shotgun rather than a 22. By the way my non technical explanation may suck but essentially those other small peaks that are close to the identity of the peptide are filtered out. Best I can do. So another lab would just include it as part of the purity and the purity would be 99pct plus.
“Simply put the other molecule would be identified as part of the peptide without a solid reference sample due to it being so close in identity”That’s from a person who supplies this equipment
This explains why freedom showed 99pct plus and Janoshik was 97
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The variance from a good quality lab using a high quality reference standard isn’t as high as you think. The difference is less than 5% and more commonly 2-3% So where you see 99.9% which is not an accurate figure it’s anywhere from 96 to 99 in that range simply because the equipment is looking at a larger range. So often you will see a peak down range outside of the filtered range.
Same person
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