Click to milligram
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How many milligram in 1 click?
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I am going to presume here you mean 1 click on a peptide injection pen similar to the v2's sold on peptide critic.
I'm also going to talk you through how getting the answer works before pointing you to the peptide calculator. The calculator is extremely helpful, but if you understand what is going on, its easier to catch your own errors.
Each click of a peptide pen represents 0.01 ml (one hundredth of a milliliter), typically called a 'unit'. The v2 can click up to 60 units, which would be 0.6 ml (60/100 milliliters).
But your question wasn't about volume (milliliters) it was about mass (milligrams).
When you receive a vial it typically has printed on the label the number of milligrams (mg) in the vial. Be cautious with this, as many vendors overfill (or, less commonly underfill) their vials. So if a vial says it contains 10mg, it might contain 12.4 mg, or 9.6 mg (or some other value). You can get a sense for what the likely mass in the vial is by looking at its COA, which will generally include that information. Its important to realize that even the COA isn't perfect on that count though, as its only measuring the vials from the batch that were provided, and so while it can be generally indicative, its not gospel. If multiple vials from the batch are reported tested on the COA, and their reported masses are close, you can be slightly more confident that the variation between the vials tested and your vial are small.
When you reconstitute a vial of peptides, you add some volume of a reconstitution fluid, typically bacteriostatic water.
This results in a concentration of peptides (mg/ml). So for the sake of example, if you had a 10mg vial and added 1 milliliter (ml) of water to the vial to reconstitute, your concentration would be: 10 mg / 1 ml = 10 mg/ml.
So how do you get to the 'click' (unit) amount in mg. Well, 1 ml = 100 units. So a concentration in mg/ml = mg/(100 units).
So in our example with a 10mg/ml concentration, that would be 10mg/ml = 10mg/(100 units) = 0.1 mg/unit.
In practice, people often work backwards from the dose they want to insure its a round number of units that is easy to remember. For example, if you wanted a 0.5 mg dose (500 micrograms(mcg)) of your peptide, and you find 10 units (10 clicks) a convenient round number, you'd want
concentration * 10 units = 0.5 mg
since 10 units is 0.1 ml that means
concentration * 0.1 ml = 0.5 mg
so
concentration = 0.5 mg/ 0.1 ml = 5 mg / ml
If your vial was 10 mg, that means you'd add 2 ml of reconstitution solution to it to your vial.
Peptide Critic has been kind enough to provide a calculator to help you do the computations:
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@ed-warnicke - Somewhat. I have to admit, hate math and, numbers really do stress me out. There is just a great deal of conflicting information that I am seeing. I have 10 mg vials of MOTS-c for my rat. I've seen reconstitute with 1 mL, 2 mL, and even 3 mL of BAC.
Say i'm going to dose my rat .5 mg once daily. Am I to add 1 mL or 2 mL to reconstitute? I really want to get ahold of the math before I go with a calculator. I think it's better to have a full understanding of concepts / principals before introducing tools to make it easier.
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@sykocis - this is a perfect example. Let me try to work this example (slowly) and see if it makes sense to you:
You want to dose your rat with 0.5mg once daily. That's your mass dosage.
But what you actually measure out on your pen or insulin syringe is a volume dosing in 'units'. Each unit is 0.01 mL.
Realistically, you probably want to dose yourself (volumetrically) in multiples of 10 units. So the question becomes: how do you make 10 units volumetrically be 0.5mg in terms of mass.
To do that you want your reconstituted solution to have a concentration of:
0.5 mg / 10 units = 0.5mg / 10 units* (0.01 mL per unit) = 0.5mg / 0.1 mL = 5 mg/mL
You have a 10 mg vial. 10 mg / 2mL = 5mg / mL. So you would want to reconstitute with 2 mL of Bac Water.
Part of what can be confusing is that you have choices you get to make:
- What mass dosage you want to target ( 0.5mg in your case )
- What volumetric dosage you want to target ( 10 units in this example).
Once you make those choices its a matter of working out the concentration that makes those two match.
Once you know your concentration you scale up to the mass in your vial to get the amount of bac water.Kudos to you for wanting to understand the math before using the calculators. It really helps in being able to 'check' to make sure your results make sense.
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OK. I understand a bit better now. I'm just going to have to create my own measures and practice. I'm still not sure why one would use 2 mL of Bac Water, unless I am completely misunderstanding that in order to attain our 5mg / mL concentration, we divided the 10 mg amount of product within our vial by 2mL (the 2mL essentially being the amount of BAC we would reconstitute with correct)? Am I overcomplicating this lol?
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I keep a spreadsheet and use it to manage and plan recons. How much to use is your choice and should be based (imo) on the vial contents, how much you want to inject, and whether high dilution is desired. If you put too much bac water in, you might be injection 4 units, which is tiny and hard to measure and use without loss. On the other hand, you might not want to be injecting 100 units if the vial is 10 mg and you are dosing 5 mgs. GHK is an example of something most researchers dilute more to avoid burning.
So it all depends. You get a lot more comfortable as you go along.
this also means that you may want to avoid buying the largest vial you can to ‘save’ money, if it means you will be injecting less than 10 units or that the vial will last 2+ months, which is not ideal. I did that with my last vial of CJC/IPA and was injecting 6 units, which was okay but I did go well past the ‘safe use’ date.