Are different filter diameters used for specific purposes? is using a larger diameter detrimental for one peptide vs another? [not sure why that would be,, but alas, is why i'm here] is even more peptide wasted with a 25 and 33mm than the 13? and lastly, is the waste referring to the liquid left in the nozzle or the membrane's capture of peptide molecule or just its absorbtion? thx for your help ++