SS-31 Dosage for Researching
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SS-31 is a mitochondrial drug. Most clinicians who use it favor morning dosing, and it’s usually approached as a time-limited intervention, especially when combined with GLP-1 agents. Given body weight and the combination being considered, this is something that really should be reviewed with a clinician familiar with mitochondrial pharmacology.
Mitochondrial and appetite signaling will already be altered by GLP-1 agonism, SS-31 modifies mitochondrial efficiency and ROS signaling, The combination can compound fatigue, hypotension, appetite suppression, or exercise intolerance.
What are your goals with your research?
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funny I was planning on dropping a few protocols from our notes today. Just posted it but here it is again.
For research use only. Not medical advice.
Overview
SS-31 (Elamipretide) is a mitochondria-targeted tetrapeptide studied for its ability to stabilize cardiolipin, improve electron transport chain efficiency, and reduce mitochondrial oxidative stress.
This protocol reflects a stepwise dose-escalation model commonly attributed to Dr. William Seeds, designed to maximize mitochondrial repair while maintaining tolerability.The approach emphasizes:
Low daily dosing first for acclimation
Moderate daily dosing next for functional improvement
Intermittent high-dose pulses to drive deeper tissue saturation
SS-31 Escalation Protocol
Phase 1 — Acclimation Phase
Duration: ~25 days
Dose: 4 mg daily
Purpose:
Introduce SS-31 gently
Allow mitochondrial membranes to adapt
Establish baseline energy and tolerance
This phase prioritizes consistency over intensity.
Phase 2 — Functional Support Phase
Duration: ~20 days
Dose: 10 mg daily
Purpose:
Increase mitochondrial ATP efficiency
Support improved energy production
Prepare mitochondria for higher intermittent exposure
By this phase, mitochondria are considered “responsive” and able to utilize higher doses more effectively.
Phase 3 — High-Dose Pulse Phase
Duration: ~6 weeks
Dose: 50 mg every third day
Total doses: ~14 administrations
Average daily exposure: ~16–17 mg/day (averaged)
Purpose:
Deliver high tissue saturation intermittently
Allow recovery days between doses
Drive cumulative mitochondrial repair
This pulsed approach is intended to “bombard” mitochondria periodically while avoiding continuous overload.
Total Protocol Duration
~8–10 weeks total
SS-31 is stopped completely after completion
This is treated as a full mitochondrial repair course, not a maintenance protocol
Cumulative SS-31 exposure in this model approaches ~1 gram total, which is considered a milestone dose in this framework.
Timing
Once daily during daily phases
Every third day during pulse phase
Time of day is flexible; consistency matters more than timing
Cycle Frequency
This protocol is not intended for continuous repetition.
Typical research patterns:
Single full course, then
Several months off before considering another cycle
Some researchers repeat annually or semi-annually depending on goals.
Common Research Notes
Energy changes are often gradual and cumulative, not immediate
Some subjects report transient fatigue early in Phase 1
The pulsed phase is considered the most impactful but also the most demanding
This protocol focuses on mitochondrial membrane repair, not AMPK activation or metabolic signaling
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Very thought provoking protocol! Thank you very much for posting this!
Delete if inappropriate, Tydes (peptides in NC) has a 60mg vial SS-31 choice still available. I snapped up a passel of them.
@PeptidePete totally allowed. Get it while you can. Full grey will be the only option soon.
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Southern Aminos is clearing out their supply…Im planning on starting a SS31 mitochondria healing before a MotsC protocol. I have been exposed to mold remodeling bathrooms in the past 15 years. My energy and recovery has dropped in this 62 year old vessel hence my peptide journey. My ultimate goal is to get back on my surfboard
and build a house on the coast -
@diegoc I condensed 7 vials into one pen for 83.5 mg and about 35 units per 10mg. But yeah, 8 is very much on the low side of worth loading into a pen. I prefer 20+. To me it’s worth it not loading the syringes.
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I just moved up to 10mg a day, 40 ish units. For the most part, no to minimal ISR’s. Sometimes a bit of redness and swelling but nothing worse than what I get from Glow. I have another 12 days at 10mg and then taking a break/switching to MOTS-C. I am skipping the pulse phase for practical and financial reasons.
I am loving the Gansulin pen for larger unit injections.
