SS-31 Dosage for Researching

diegoc

For 10MG vials i dont think its worth putting it in a pen

diegoc

If you have those 10MG vials put 2 of them together i did that for mine

ResearchCat

@diegoc I condensed 7 vials into one pen for 83.5 mg and about 35 units per 10mg. But yeah, 8 is very much on the low side of worth loading into a pen. I prefer 20+. To me it’s worth it not loading the syringes.

PeptideEd

@diegoc For 10mg vials I put 3 vials in a pen for the 4mg/day dose

PeptideEd

@diegoc Any ISR at that concentration? Also... SS-31 isn't blue for me... is that GHKCU?

diegoc

No its not a blue peptide its the shadow of the pen

PeptideEd

@diegoc Got it... so no ISR at that concentration?

diegoc

At 4mg daily no ISR only the first day 2 weeks ago were a weird welt came out and disappeared right away.

Im doing this exact ss-31 protocol which seems to be really good

ResearchCat

I just moved up to 10mg a day, 40 ish units. For the most part, no to minimal ISR’s. Sometimes a bit of redness and swelling but nothing worse than what I get from Glow. I have another 12 days at 10mg and then taking a break/switching to MOTS-C. I am skipping the pulse phase for practical and financial reasons.

I am loving the Gansulin pen for larger unit injections.

diegoc

I just ordered 3 Gansulin pens, i cant wait to add SS-31 for when im on the higher dose.

PeptideEd

I've now switched over to adding 0.6 ml to a 30 mg vial of SS-31 for a 50mg/ml concentration. At that concentration:

20 units = 10 mg

I injected 20 units last night and did not experience any ISR at all. For those (like me) who get nervous about high concentrations... thought you'd like to know.

MacLeeezy

Any thoughts on the recent study: Gudiksen et al., Free Radical Biology and Medicine (2026) that talks about potentially running SS31 and Mots-c together instead of Ss31 first and then Motsc? Josh Holyfield posted a video about it that was interesting on YouTube. https://m.youtube.com/watch?v=Arx8CD6TA0Y&pp=ygU5R3VkaWtzZW4gZXQgYWwuLCBGcmVlIFJhZGljYWwgQmlvbG9neSBhbmQgTWVkaWNpbmUgKDIwMjYp

Just curious how your mice prefer to run both? Together or staggered?

diegoc

im in the 2nd phase of ss-31 and i added Mots-C

PeptidePete

@MacLeeezy said in SS-31 Dosage for Researching:

Any thoughts on the recent study: Gudiksen et al., Free Radical Biology and Medicine (2026) that talks about potentially running SS31 and Mots-c together instead of Ss31 first and then Motsc?

Just curious how your mice prefer to run both? Together or staggered?

I have only ever considered running both at the same time. Complimentary in every way. No such thing as "repair" then "grow" in my point of view, silly concept.

My stack: SS-31/NAD+/MOTS/5-AMINO to start, titrated up in frequency/dose, then when I completed and discontinued the SS-31 50mg per day for 60 days, added O-304/SLU/BAM-15 to the others. LOVE IT. Keep in mind, I am very well optimized in general, so working on the top of the pyramid. Not the stack for everybody depending upon individual sensitivities.

Freebie- if you are serious about optimizing mitochondria, you darn well better be incorporating the very best EPA/DHA/DPA sources, specifically fish eggs, preferably sturgeon (suggest the reader research it as I did). The fatty acids are taken up into the all your cell membranes over time. It's freaking crazy stuff, search it and learn it. No one ever talks about this...except me. A "superpower" addition to a serious and effective mitochondrial stack. You're welcome

PeptideEd

@peptideed do you have a preferred source of sturgeon eggs?

PeptidePete

@PeptideEd said in SS-31 Dosage for Researching:

@peptideed do you have a preferred source of sturgeon eggs?

Yeah...you have to let your wallet be your guide on this one In nutritional ranked order (seriously, big differences):

Sturgeon- I look for 1kg tins which is where best value per gm/oz is found and last me 1.5-2 weeks per tin in spite of the suggestion they be used in days. I keep the tin(s) in a vacuum bag except when serving. Look for discounts which is what I do. I personally like Bester, but others are good and can be found on Amazon.

Paddlefish is an alternative but releases oil in the tin and flavor/texture not as nice, price is approx -50% vs sturgeon.

Salmon roe is even lower cost per gm/oz but definitely an acquired taste, buy the wild ones shipped frozen!

Trout/flying fish roe is wholly inferior nutritional quality, I would not use.

Repair...Optimize (roe)...Uncouple. My big picture protocol.

This approach I'm sure sounds batshit crazy to some, but serious researchers will understand.

MacLeeezy

@PeptidePete right on. I appreciate the feedback. Time to do my sturgeon homework!

PeptideEd

@peptidepete Shopping at Bester... is there any particular variety they sell you'd recommend?

PeptidePete

@PeptideEd said in SS-31 Dosage for Researching:

@peptidepete Shopping at Bester... is there any particular variety they sell you'd recommend?

A couple steps up from "the bottom", as finances allow. Better flavor, texture, coloration, roe size, and not as much oil comes out and collects in the bottom of the tin (some may enjoy this or not care in exchange for better "value").

All the same nutrient benefits accrue regardless of species as long as it is sturgeon, just becomes a matter of sensory appreciation.

Mitochondrial optimization is so multi-faceted, it is a lot of fun to explore and research. A shit-load more to it than just "running 2mg SS-31 every few days"...haha I am going in for labs (again) this Friday AM, and two weeks ago submitted my second quarterly TruDiagnostic blood sample for methylation and telomere testing and all the other stuff they do. BTW I have been very hard-core carnivore for 2 3/4 years now.

ResearchCat

@peptidepete I love everything about you recommending caviar to supplement my peptide research. I think this year’s barbeque is going to feature a ‘metabolic enhancement’ table along with 8 different kinds of smoked meat.