SS-31 Dosage for Researching

beachfun

Southern Aminos is clearing out their supply…Im planning on starting a SS31 mitochondria healing before a MotsC protocol. I have been exposed to mold remodeling bathrooms in the past 15 years. My energy and recovery has dropped in this 62 year old vessel hence my peptide journey. My ultimate goal is to get back on my surfboard and build a house on the coast

PeptideEd

@randy any guidance on concentrations for the various steps? For 4mg per day I'm using 10mg/ml which works out to a doable 40 units. For 10mg/day or 50mg/day presumably one moves to higher concentrations... recommendations?

diegoc

How many MG is the bottle of SS-31

PeptideEd

@diegoc good question... for my 4mg/day run, I was using 10mg vials. For the 10mg/day and 50mg/day I'll be using 30mg vials.

diegoc

diegoc

For 10MG vials i dont think its worth putting it in a pen

diegoc

If you have those 10MG vials put 2 of them together i did that for mine

ResearchCat

@diegoc I condensed 7 vials into one pen for 83.5 mg and about 35 units per 10mg. But yeah, 8 is very much on the low side of worth loading into a pen. I prefer 20+. To me it’s worth it not loading the syringes.

PeptideEd

@diegoc For 10mg vials I put 3 vials in a pen for the 4mg/day dose

PeptideEd

@diegoc Any ISR at that concentration? Also... SS-31 isn't blue for me... is that GHKCU?

diegoc

No its not a blue peptide its the shadow of the pen

PeptideEd

@diegoc Got it... so no ISR at that concentration?

diegoc

At 4mg daily no ISR only the first day 2 weeks ago were a weird welt came out and disappeared right away.

Im doing this exact ss-31 protocol which seems to be really good

ResearchCat

I just moved up to 10mg a day, 40 ish units. For the most part, no to minimal ISR’s. Sometimes a bit of redness and swelling but nothing worse than what I get from Glow. I have another 12 days at 10mg and then taking a break/switching to MOTS-C. I am skipping the pulse phase for practical and financial reasons.

I am loving the Gansulin pen for larger unit injections.

diegoc

I just ordered 3 Gansulin pens, i cant wait to add SS-31 for when im on the higher dose.

PeptideEd

I've now switched over to adding 0.6 ml to a 30 mg vial of SS-31 for a 50mg/ml concentration. At that concentration:

20 units = 10 mg

I injected 20 units last night and did not experience any ISR at all. For those (like me) who get nervous about high concentrations... thought you'd like to know.

MacLeeezy

Any thoughts on the recent study: Gudiksen et al., Free Radical Biology and Medicine (2026) that talks about potentially running SS31 and Mots-c together instead of Ss31 first and then Motsc? Josh Holyfield posted a video about it that was interesting on YouTube. https://m.youtube.com/watch?v=Arx8CD6TA0Y&pp=ygU5R3VkaWtzZW4gZXQgYWwuLCBGcmVlIFJhZGljYWwgQmlvbG9neSBhbmQgTWVkaWNpbmUgKDIwMjYp

Just curious how your mice prefer to run both? Together or staggered?

diegoc

im in the 2nd phase of ss-31 and i added Mots-C

PeptidePete

@MacLeeezy said in SS-31 Dosage for Researching:

Any thoughts on the recent study: Gudiksen et al., Free Radical Biology and Medicine (2026) that talks about potentially running SS31 and Mots-c together instead of Ss31 first and then Motsc?

Just curious how your mice prefer to run both? Together or staggered?

I have only ever considered running both at the same time. Complimentary in every way. No such thing as "repair" then "grow" in my point of view, silly concept.

My stack: SS-31/NAD+/MOTS/5-AMINO to start, titrated up in frequency/dose, then when I completed and discontinued the SS-31 50mg per day for 60 days, added O-304/SLU/BAM-15 to the others. LOVE IT. Keep in mind, I am very well optimized in general, so working on the top of the pyramid. Not the stack for everybody depending upon individual sensitivities.

Freebie- if you are serious about optimizing mitochondria, you darn well better be incorporating the very best EPA/DHA/DPA sources, specifically fish eggs, preferably sturgeon (suggest the reader research it as I did). The fatty acids are taken up into the all your cell membranes over time. It's freaking crazy stuff, search it and learn it. No one ever talks about this...except me. A "superpower" addition to a serious and effective mitochondrial stack. You're welcome

PeptideEd

@peptideed do you have a preferred source of sturgeon eggs?