AOD-9604 Research Dosing Protocol
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AOD-9604 is a modified C-terminal fragment of human growth hormone (177–191) commonly researched for its lipolytic and anti-lipogenic properties.
It does not meaningfully affect IGF-1 in human studies and tends to have a mild, well-tolerated profile.
Most research models use once-daily subcutaneous administration.Daily Dosing Protocol (Subcutaneous)
Standard Daily Range
YOUR RAT MUST BE FASTED 4 hours prior and 2 hours post injection
200–500 mcg once daily
Administered at approximately the same time each day.
Titration Schedule
Weeks 1–2
200 mcg daily
Establishes baseline tolerability.
Weeks 3–6
400 mcg daily
Mild step-up for increased activity.
Weeks 6–12 (OPTIONAL)
500 mcg daily
Often considered the “working dose.”
Common upper-end daily dose used in longer metabolic research cycles.
Cycle Length
Typical AOD-9604 research cycles:
6–12 weeks on protocol
Optional 2–4 week break before repeating, depending on research goals.
AOD is mild enough that some researchers run it continuously, but cyclical patterns are still common.
Alternate Approaches
Twice-Daily Split
200–300 mcg twice daily
Used when the goal is more constant lipolytic signaling throughout the day.
Training-Day Bias
Dose in the morning or pre-training, depending on preference.
No consensus on timing — daily consistency matters more.
Common Research Notes
AOD-9604’s effects tend to be subtle and cumulative rather than acute.
Works best when paired with caloric control or increased physical activity.
Side effects are minimal; occasional mild flushing or warmth at the injection site may occur.
Reconstitution Instructions
https://www.youtube.com/watch?v=U5i_g6ZhAU0 -
Curious, I have a pretty good stash of this but Most users say they haven't noticed any results. Does Randy use it?
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I have used it, and I still have a fair amount on ice.
Around 500 mcg is where I noticed an effect. The scale started moving again, but in hindsight I probably would have been better off simply increasing my Survo or Tirz dose at that point for a more predictable result. I have heard from others who claim they did not notice much until they stacked it with 5-amino or NAD and pinned them at the same time, supposedly as a “carrier,” but that feels more like bro science than anything evidence-based.
In my experience, this is a “last few pounds” compound. It is not something I would stack with a GLP-1 when you are still early in the process.
I am also unusually sensitive to peptides. BPC-157/TB-4 gives me full-body itching. Retatrutide was significantly worse. AOD, on the other hand, caused no issues for me.
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Interesting, thanks for the reply, I really appreciate it and this site overall. That’s exactly how I was thinking of using it (at the end of the weight loss) to help with defining abs and clean out the last bit of fat in the belly area. My mouse is on a blood thinner so the choices are slim. AOD was one pep I can try that doesn’t interact with it. Keep up the great info you provide, it’s been invaluable to me as a relatively new researcher.
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Generally speaking, drinking black coffee shouldn’t affect your blood chemistry wrt fasting/sugar.
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It shouldnt but some doctors (the ones with PhD's) argue that it does.
IDGAF. Randy needs his quad shot with his tesamorelin
@Randy yeah, not to play the ‘my doctor can beat up your doctor’ game, but my doctor/hospital says it is okay to have black coffee before labs and I haven’t seen any difference in results due to that. I’d be curious to see the rationale behind arguing that coffee affects your insulin or fasting state. Haven’t found much to that but I guess it’s another rabbit hole to go down.
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Thanks for all the info, guys. My Kangaroo appreciate this community since I'm all new to this stuff.
Reconstitution question: I got 2mg vials of AOD 9604 and wondering if I should add 1ml Bac water or 2ml ? Any suggestion would be helpful.
regarding daily dosage 0.3 MG daily early morning?
8 to 12 weeks cycle ? -
.2-.5 mg. Start with .2 or lower to test tolerance and make sure you dont have a reaction.
As far as reconstituting https://www.youtube.com/watch?v=U5i_g6ZhAU0
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I started using AOD 2 weeks ago and I definitely have dropped inches. Especially in my thighs and arms which for me are my hardest areas to lose fat. I started slow and now I’m up to 30units(1.5mg). So far pleased with the results. On a bonus note I also am experiencing less knee pain. I have OA and since starting AOD I find I have less pain and can do far more PT exercise during my sessions.
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Theres a Doc on Youtube that really loves AOD on Tirz..
Its a shame Jeff Reta gives you the itches, same as BPC and TB…
How about MOTS-C
@diegoc aside from some sting MOTS-C doesnt really bother me...though i fear one day ill be a lucky recipient of the allergic reaction a number of people report. Im off mots at the moment. too many stacked as it is...but im staring down a kit in my fridge every time i reach for a white monster.
The reta itches....hard pass. Im going to be interested to see what work arounds there are once it passes and goes mainstream.
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@Randy the AOD I got was from verified peptides and is 10mg. I reconstituted with 2mL Bac water. I figured the dosing based on that info. 30 units = 1.5 mg. Haven’t had any untoward SE with that dose.
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@diegoc aside from some sting MOTS-C doesnt really bother me...though i fear one day ill be a lucky recipient of the allergic reaction a number of people report. Im off mots at the moment. too many stacked as it is...but im staring down a kit in my fridge every time i reach for a white monster.
The reta itches....hard pass. Im going to be interested to see what work arounds there are once it passes and goes mainstream.
