We tested peptide degradation so people can stop guessing
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Thank you for completing this investigation, it is very informative for research purposes! This type of information just isn't typically available, so I really appreciate your investment on all our behalf. Kudos to you & the Rat!
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I'm curious to see the sharp drop after a few months for Tirz even in the fridge. How does the pharma pen stay valid for their expiry dates?
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@bangkokiscool yeah, I was thinking of this, but it is worth noting that compounded tirz with the ‘extended BUD’ has preservatives in it to help it remain stable and viable. So it would likely require a separate test using (very expensive) vials sourced from a compounding pharmacy.
I have received compounded Tirz from two different compounding pharmacies, one of whom regularly sent product that was past its BUD with a note showing that their testing shows it is good for 6 months after that and and extended BUD.
I can only attest from using it that it still works, but idk what the potency was.
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And yeah, they never provide either test results or vial potency/concentrations.
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a beautiful post! thank you for that you guys and rodents are the best Clouse Salutes you
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@randy Not that I'm questioning you, but in the interest of openness I'm curious if your actual test result docs state that this was reconstituted vials tested. The reason I ask is there are gymbros on dudetube saying the same things, but the test results (in one particular example from Janoshik) say nothing about whether the test was on reconstituted peptide or just lyophilized. Again not saying you are being dishonest, but the other guy's test results were definitely ambiguous.
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@randy Not that I'm questioning you, but in the interest of openness I'm curious if your actual test result docs state that this was reconstituted vials tested. The reason I ask is there are gymbros on dudetube saying the same things, but the test results (in one particular example from Janoshik) say nothing about whether the test was on reconstituted peptide or just lyophilized. Again not saying you are being dishonest, but the other guy's test results were definitely ambiguous.
@kj4otu If your read this post he clearly spell it out "one diluent type"
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@kj4otu If your read this post he clearly spell it out "one diluent type"
We partnered with Analytical Formulations Inc. (AFI) to evaluate the stability of three peptides after reconstitution:
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@randy Not that I'm questioning you, but in the interest of openness I'm curious if your actual test result docs state that this was reconstituted vials tested. The reason I ask is there are gymbros on dudetube saying the same things, but the test results (in one particular example from Janoshik) say nothing about whether the test was on reconstituted peptide or just lyophilized. Again not saying you are being dishonest, but the other guy's test results were definitely ambiguous.
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I'm curious to see the sharp drop after a few months for Tirz even in the fridge. How does the pharma pen stay valid for their expiry dates?
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I'm curious to see the sharp drop after a few months for Tirz even in the fridge. How does the pharma pen stay valid for their expiry dates?
@bangkokiscool Pharmaceuticals pens and compounding have a lot of additives to stabalize. IVMedco compounded Tirzepatide for example is Tirz, Vitamin B12, Sodium Chloride, Sodium Dibasic Heptahydrate and Water plus HCL to balance ph if needed.
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@daring Not really — the fridge samples in the study sat the entire time at room temp and showed little drop by day 15 even. The brief temperature excursion from pulling a vial out, drawing a dose, and putting it back takes maybe 30–60 seconds and barely moves the needle on cumulative thermal exposure. If if you let it warm up to room temp before putting it back i thats only hours of time at room temp over the course of a cart, The bigger drivers are overall storage temperature and time.
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@daring I was curious about the same thing so ran it through AI and here what was reported:
"One stability guide notes that if reconstituted MOTS‑c is kept at 4 °C continuously, it stays >95% pure for about 28 days; intermittent warm excursions accelerate loss, dropping to ~85% by 14 days and <70% by 28 days when repeatedly warmed to room temperature."
This correlates somewhat with what Jeff reported on his very awesome study (Thank You Jeff!) although his study reported more degradation at 31 days than sighted here.
Nonetheless, given enough correlation I think I can assume with a high degree of confidence based on both sources of info that yes I could expect MOTS C to degrade considerably more warming to room temperature for injection rather than continuously refrigerating.
I also asked AI about injecting sub peptide at 35 degrees F...
"For a typical subcutaneous peptide injection (like 0.25–0.5 mL in belly fat), having the solution very cold mainly increases local discomfort and the chance of a small lump or irritation, but it’s unlikely to cause deep or systemic damage by itself."
Based on all of this, I think I'll refrigerate of course, take my pen straight away and inject sub then put peptidecritic pen back into the frig. Not recommending this to anyone else but that's what I think I'll do for my rat. Of course it depends on lots of different variables not the least of which how long you expect your vial to be stored before being fully dispensed into your rat.
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@kj4otu "All samples in this study were prepared using bacteriostatic water with 0.9% benzyl alcohol."
It's sprinkled all throughout the post.
If we didn't reconstitute them, the tests would be worthless. There would also be no degradation of any kind.
@Randy I get that you said it multiple times. I am asking about the actual coa document/lab results from the testing lab. I would like to see that posted and would like to see it state the actual test parameter. Like I said, I’m not trying to question your statement just asking for verification. Others posting these same comments in testing have also not shown the actual test results from the lab. Your stuff seems different/more legit so I assumed you would jump at the chance to show lab verification. No offense but I don't actually know you. Trust but verify you know. Again not trying to just make waves here.
