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Peptide Critic Community

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  1. Randy the Rats Research Forum
  2. Supplies, Mixing & Storage
  3. What gauge breather needle to purchase?
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What gauge breather needle to purchase?

Scheduled Pinned Locked Moved Supplies, Mixing & Storage
syringespenscartridgesfilters
14 Posts 6 Posters 121 Views
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  • R Online
    R Online
    ResearchCat
    wrote last edited by
    #5

    I use 30/31g

    Please set a funny and sarcastic signature line. It brings me joy. Thank you for your attention in this matter.

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    • R Online
      R Online
      ResearchCat
      wrote last edited by
      #6

      @vpeptides The problem with that is blowing the plug out of the cartridge. Are you filling carts without a vent and not having the plug come out? That seems unlikely to me.

      Please set a funny and sarcastic signature line. It brings me joy. Thank you for your attention in this matter.

      vpeptidesV 1 Reply Last reply
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      • R ResearchCat

        @vpeptides The problem with that is blowing the plug out of the cartridge. Are you filling carts without a vent and not having the plug come out? That seems unlikely to me.

        vpeptidesV Offline
        vpeptidesV Offline
        vpeptides
        wrote last edited by
        #7

        @ResearchCat I was talking about a 3ml vial, not a cartridge. Didn't read the post carefully. I am a writer, not a reader, you know? 🙂

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        • vpeptidesV vpeptides

          I don' use a breather needle. I inject the liquid through the filter, then detach the filter from the needle to let the extra air out.

          E Online
          E Online
          Eleanor
          wrote last edited by
          #8
          This post is deleted!
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          • vpeptidesV vpeptides

            I don' use a breather needle. I inject the liquid through the filter, then detach the filter from the needle to let the extra air out.

            E Online
            E Online
            Eleanor
            wrote last edited by
            #9

            @vpeptides How do you tell when all the air is out of the vial?

            vpeptidesV 1 Reply Last reply
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            • R Online
              R Online
              ResearchCat
              wrote last edited by
              #10

              @vpeptides All good! Thanks for clarifying. That makes sense.

              Please set a funny and sarcastic signature line. It brings me joy. Thank you for your attention in this matter.

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              • E Eleanor

                @vpeptides How do you tell when all the air is out of the vial?

                vpeptidesV Offline
                vpeptidesV Offline
                vpeptides
                wrote last edited by
                #11

                @Eleanor it happens momentarily

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                • vpeptidesV vpeptides

                  @Eleanor it happens momentarily

                  E Online
                  E Online
                  Eleanor
                  wrote last edited by
                  #12

                  @vpeptides Ok, does it hiss, pop, any indication?

                  vpeptidesV 1 Reply Last reply
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                  • E Eleanor

                    @vpeptides Ok, does it hiss, pop, any indication?

                    vpeptidesV Offline
                    vpeptidesV Offline
                    vpeptides
                    wrote last edited by
                    #13

                    @Eleanor Long story: It hisses when I slightly release the needle from the filter to equalize the pressure. Then I reconnect it and disconnect the filter from the syringe to add more air into it, then reconnect again and push to let whatever is still in filter to go through. Then disconnect again the needle.

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                    • ? Offline
                      ? Offline
                      A Former User
                      wrote last edited by
                      #14

                      Thanks all! So excited to get started with filtering and pens!

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