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Peptide Critic Community

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  1. Randy the Rats Research Forum
  2. Supplies, Mixing & Storage
  3. Thawing Frozen Peptide
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Thawing Frozen Peptide

Scheduled Pinned Locked Moved Supplies, Mixing & Storage
storagestorage-temprefrigerationbeginner-question
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  • A Offline
    A Offline
    a.arrington
    wrote last edited by
    #1

    I try to keep approximately an 8-12 month supply on hand of various peptides. I freeze them until ready to use. My question is about thawing the vial out. Normally I put the vial in a box in a dark cabinet and then reconstitute it. Should I be thawing them out in the fridge?

    pep_researcherP 1 Reply Last reply
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    • NewenoughN Offline
      NewenoughN Offline
      Newenough
      wrote last edited by Newenough
      #2

      @a.arrington I'd recommend a search on the site, as we've covered this topic recently and in great detail. But, if you're only looking at storing 8-12 months worth of working product, freezing is not required or even recommended. Freeze thaw cycles are bad for peptides and moisture ingress into the vials from condensation is a huge risk. Best solution for 12 month or less (short term storage) is to use an air tight flask, such as the hydropeak (Amazon). 3 sizes to choose from. You can pickup inserts for it (also Amazon) or 3d pint your own if you like. The air tight container mitigates temp swing in the fridge and door opening and closing. When you need another vial, take it out and allow it to warm up slowly to room temp. I give mine over night as the containers are thermally awesome. This prevents temp shocks and moisture when opening. Also, you'll want to include desiccant packs in there as well. You can print or buy little vialed shape holders for it. You can find 2-5lb jugs of reusable desiccant pellets on Amazon for cheap, and last you a life time.

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      • A a.arrington

        I try to keep approximately an 8-12 month supply on hand of various peptides. I freeze them until ready to use. My question is about thawing the vial out. Normally I put the vial in a box in a dark cabinet and then reconstitute it. Should I be thawing them out in the fridge?

        pep_researcherP Online
        pep_researcherP Online
        pep_researcher
        wrote last edited by pep_researcher
        #3

        @a.arrington said:

        Should I be thawing them out in the fridge?

        No. I keep mine in freezer regardless of when I'd use them.

        Just before recon, take it out, let it sit at room temp for about 30 mins. Then recon. No issues.

        I don't refrigerate my BAC water. It stays in a dark shelf in my pantry in a ziplock. If you refrigerate BAC, then it needs to be at room temp too. I personally don't recommend refrigerating BAC to avoid these cold and warm cycles, and I don't throw it away at the drop of 30th day. Have used it for 90-100 days without any problems, with strict asceptic handling (no repoking a noodle once it's out of BAC bottle).

        E 1 Reply Last reply
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        • pep_researcherP pep_researcher

          @a.arrington said:

          Should I be thawing them out in the fridge?

          No. I keep mine in freezer regardless of when I'd use them.

          Just before recon, take it out, let it sit at room temp for about 30 mins. Then recon. No issues.

          I don't refrigerate my BAC water. It stays in a dark shelf in my pantry in a ziplock. If you refrigerate BAC, then it needs to be at room temp too. I personally don't recommend refrigerating BAC to avoid these cold and warm cycles, and I don't throw it away at the drop of 30th day. Have used it for 90-100 days without any problems, with strict asceptic handling (no repoking a noodle once it's out of BAC bottle).

          E Offline
          E Offline
          Eleanor
          wrote last edited by
          #4

          @pep_researcher

          A really interesting process you have there about keeping the powder frozen until reconstitution time. It makes a lot of sense and I think I'll adopt it. Thank you!

          With regard to the BAC, I guess I feel better keeping it in the fridge (once punctured), surrounded by absorbers and stored in a proper container. I take the BAC out, take the amount I need (while cold) and return the BAC into fridge storage and let the syringes warm up. If the BAC is unused, I store in room temp dark container, with lots of absorbers. It seems to work well.

          pep_researcherP 2 Replies Last reply
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          • E Eleanor

            @pep_researcher

            A really interesting process you have there about keeping the powder frozen until reconstitution time. It makes a lot of sense and I think I'll adopt it. Thank you!

            With regard to the BAC, I guess I feel better keeping it in the fridge (once punctured), surrounded by absorbers and stored in a proper container. I take the BAC out, take the amount I need (while cold) and return the BAC into fridge storage and let the syringes warm up. If the BAC is unused, I store in room temp dark container, with lots of absorbers. It seems to work well.

            pep_researcherP Online
            pep_researcherP Online
            pep_researcher
            wrote last edited by
            #5
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            • E Eleanor

              @pep_researcher

              A really interesting process you have there about keeping the powder frozen until reconstitution time. It makes a lot of sense and I think I'll adopt it. Thank you!

              With regard to the BAC, I guess I feel better keeping it in the fridge (once punctured), surrounded by absorbers and stored in a proper container. I take the BAC out, take the amount I need (while cold) and return the BAC into fridge storage and let the syringes warm up. If the BAC is unused, I store in room temp dark container, with lots of absorbers. It seems to work well.

              pep_researcherP Online
              pep_researcherP Online
              pep_researcher
              wrote last edited by pep_researcher
              #6

              @Eleanor said:

              I feel better keeping it in the fridge (once punctured),

              There was some old study by big pharma or Pfizer don't recall, showing that benzyl alcohol potency and efficacy to thwart germ growth is better at room temp. Cold temp reduces the efficiency and it may also separate out in the water.

              I've been reconning peps now for over 6 months, never stored BAC in refrigerator and haven't had any issues.

              Reconstituted peptides need to be refrigerated because now there's an amino acid chain in it and it's degradable although it can still stay good at room temp for a few days, like branded tirzepatide can stay good for 21 days.

              E 1 Reply Last reply
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              • pep_researcherP pep_researcher

                @Eleanor said:

                I feel better keeping it in the fridge (once punctured),

                There was some old study by big pharma or Pfizer don't recall, showing that benzyl alcohol potency and efficacy to thwart germ growth is better at room temp. Cold temp reduces the efficiency and it may also separate out in the water.

                I've been reconning peps now for over 6 months, never stored BAC in refrigerator and haven't had any issues.

                Reconstituted peptides need to be refrigerated because now there's an amino acid chain in it and it's degradable although it can still stay good at room temp for a few days, like branded tirzepatide can stay good for 21 days.

                E Offline
                E Offline
                Eleanor
                wrote last edited by
                #7

                @pep_researcher said:

                There was some old study by big pharma or Pfizer don't recall, showing that benzyl alcohol potency and efficacy to thwart germ growth is better at room temp. Cold temp reduces the efficiency and it may also separate out in the water.

                Really? My BAC is used within 60 days. Would it separate in that time frame? I also keep my apt. rather warm and it worries me as that it's about 80 degrees or so. Too warm?

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