IS HOSPIRA BAC WATER REALLY THE ONLY OPTION?
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If you have a link to the 12 sample BAC Water test you are referring to,would you mind sharing it ?
Interesting idea with the home brew BAC Water but unless you have a CGMP certified clean room in your home, there are so many points of potential contamination in most home environments, that I think it would be safer to use the Lambda water.
I use Hospira BAC for about 90+% of my reconstitutions. When I on occasion run out, I do use some Lamba which I have on hand, and have not had any issues.
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Distilled water is not the same as sterile water. Sterile water is ensured to have extremely low threshold of endotoxins, pH around 5.5, and specific conductivity.
If you can ensure all that for your 50cent Walmart distilled water, sure go ahead.
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Guys, be assured that making purified water is no easy task. Contaminates enter the system from everywhere. Components must be cleaned cyclically with 138+ degreeF caustic soda and/or 30% H2O2 - dangerous. Ions must be removed. pH must be controlled. Every component possible must be made from high purity teflon. I assure you that Hospira does not fire up their still and squirt steam and water into bottles. I know this from the semiconductor chemical industry. The chemical engineers are kept very busy making something as stupid as water.
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Guys, be assured that making purified water is no easy task. Contaminates enter the system from everywhere. Components must be cleaned cyclically with 138+ degreeF caustic soda and/or 30% H2O2 - dangerous. Ions must be removed. pH must be controlled. Every component possible must be made from high purity teflon. I assure you that Hospira does not fire up their still and squirt steam and water into bottles. I know this from the semiconductor chemical industry. The chemical engineers are kept very busy making something as stupid as water.
@kenhnsy You can buy purified water and mix it with benzyl alcohol. You don't need to make it; it's pretty cheap by the gallon. It is what the testing labs use to test peptides and what Hospira mixes with Benzyl Alcohol. I have not done this, but I know people who have. They have also gotten it tested for PH, sterility, and content because they were thinking of manufacturing it.
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@kenhnsy You can buy purified water and mix it with benzyl alcohol. You don't need to make it; it's pretty cheap by the gallon. It is what the testing labs use to test peptides and what Hospira mixes with Benzyl Alcohol. I have not done this, but I know people who have. They have also gotten it tested for PH, sterility, and content because they were thinking of manufacturing it.
@kenhnsy You can buy purified water and mix it with benzyl alcohol. You don't need to make it; it's pretty cheap by the gallon. It is what the testing labs use to test peptides and what Hospira mixes with Benzyl Alcohol. I have not done this, but I know people who have. They have also gotten it tested for PH, sterility, and content because they were thinking of manufacturing it.
I wish I had a shake my head emoji.
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@kenhnsy You can buy purified water and mix it with benzyl alcohol. You don't need to make it; it's pretty cheap by the gallon. It is what the testing labs use to test peptides and what Hospira mixes with Benzyl Alcohol. I have not done this, but I know people who have. They have also gotten it tested for PH, sterility, and content because they were thinking of manufacturing it.
@Stevepep You can do that with cell culture lab grade sterile water. It's not NDC so doesn't need script, dirt cheap, and has almost the same qualifications as pin grade sterile water.
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To be clear, Hospira is my first choice. Genetek is my second.
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@Stevepep You can do that with cell culture lab grade sterile water. It's not NDC so doesn't need script, dirt cheap, and has almost the same qualifications as pin grade sterile water.
@pep_researcher said:
https://www.thermofisher.com/order/catalog/product/A1287304?SID=srch-srp-A1287304.Was thinking about this.
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@mmp I throw it out everymonth since I am only using 10ML's not 30's
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@pep_researcher said:
https://www.thermofisher.com/order/catalog/product/A1287304?SID=srch-srp-A1287304.Was thinking about this.
@Stevepep Yup that's the one I shortlisted too but I don't like that it has such a wide open mouth.
One way I thought is — draw using luer lock, filter into a sealed sterile vial, add benzyl alcohol 10 units for every 10ml.
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@mmp used till last drop for 100 days with the following strict rule — once a noodle is out of BAC bottle no repoking with the same one. Fresh noodle for every poke. At times after reconstituting multiple peps, I'm left with a pile of 3ml luer locks to dispose but hey luer locks are dirt cheap.
Use noodles liberally and BAC judiciously!
My 2c.
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