COA review
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I was wondering if there is any researchers in here that could decipher this COA a bit better than provided in the email the lab sent to me. To summarize, I had a 5 mg vial of slupp332 tested. It came back as a failure, with the explanation being, "while it did absorb UV light, it is not a peptide". My problem is, slupp332 is not a peptide, so of course it would not be present in the sample. If testing ended because a peptide was not present, this could then be a flawed test, correct? I am waiting on a confirmation of this from the lab, but was wondering if there is something else I should be asking?
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Expected Absorbance Profile Overview
Wavenumber Range (cm⁻¹) Peak Shape Intensity Molecular Assignment 3200 - 3400 Very Broad Strong Phenolic -OH stretch 3150 - 3250 Sharp (Overlap) Medium Hydrazide -NH stretch 3030 - 3080 Sharp Weak Aromatic C-H stretch 1640 - 1670 Sharp Very Strong Amide C=O stretch (Amide I) 1600 - 1620 Sharp Strong Imine C=N stretch 810 - 830 Sharp Strong para-substituted Benzene ring Summary Chart Behavior
An actual scan of pure SLU-PP-332 will display a relatively quiet baseline from 4000 to 3500 cm⁻¹. It will then rise into a wide, mountain-like envelope with a sharp tooth around 3300 cm⁻¹. After dropping back down, it presents a dramatic cluster of tall, tightly grouped, needle-thin peaks. This cluster starts with the massive Carbonyl line at ~1650 cm⁻¹ and is followed by a dense fingerprint layout stretching down to 600 cm⁻¹.
(you can guess I used AI, I am not an expert in it. But this description doesn't fit your chart)
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Expected Absorbance Profile Overview
Wavenumber Range (cm⁻¹) Peak Shape Intensity Molecular Assignment 3200 - 3400 Very Broad Strong Phenolic -OH stretch 3150 - 3250 Sharp (Overlap) Medium Hydrazide -NH stretch 3030 - 3080 Sharp Weak Aromatic C-H stretch 1640 - 1670 Sharp Very Strong Amide C=O stretch (Amide I) 1600 - 1620 Sharp Strong Imine C=N stretch 810 - 830 Sharp Strong para-substituted Benzene ring Summary Chart Behavior
An actual scan of pure SLU-PP-332 will display a relatively quiet baseline from 4000 to 3500 cm⁻¹. It will then rise into a wide, mountain-like envelope with a sharp tooth around 3300 cm⁻¹. After dropping back down, it presents a dramatic cluster of tall, tightly grouped, needle-thin peaks. This cluster starts with the massive Carbonyl line at ~1650 cm⁻¹ and is followed by a dense fingerprint layout stretching down to 600 cm⁻¹.
(you can guess I used AI, I am not an expert in it. But this description doesn't fit your chart)
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Something like that should be reported (transmittance is reverse absorbance)
It's kind of close, but you are right, the numbers are off. Unless their equipment is miscalibrated.
@vpeptides ok, thanks again!
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@vpeptides The waves seem to fall inline with the explanation of how they should look, but certainly not within the numerical parameters listed. Thanks for the help! I am way to old to understand the use of AI, lol.
@crash2127 "AI" is easy
I just used Google search in "AI Mode" and asked
How should FTIR absorbance/frequency chart look for SLU-PP-332? -
@crash2127 "AI" is easy
I just used Google search in "AI Mode" and asked
How should FTIR absorbance/frequency chart look for SLU-PP-332?@vpeptides lesson learned, good to know.
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