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Freedom Again

Scheduled Pinned Locked Moved Peptide Discussion
45 Posts 13 Posters 1.1k Views 1 Watching
Freedom Again Freedom Again
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  • T Offline
    T Offline
    TXLabRat
    wrote last edited by
    #41

    Freedom gives the vendor what they want.

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    • RandyR Randy

      https://community.peptidecritic.com/topic/3540/discussion-blackline-scientific

      https://peptidecritic.com/vendor/blackline-scientific

      They list "the collective" on "their tests" when they are available. Its so lazy I cant even.

      D Offline
      D Offline
      denti
      wrote last edited by denti
      #42

      @Randy said:

      https://community.peptidecritic.com/topic/3540/discussion-blackline-scientific

      https://peptidecritic.com/vendor/blackline-scientific

      They list "the collective" on "their tests" when they are available. Its so lazy I cant even.

      Everyone should check out Randy's links for Blackline Scientific. You will certainly enjoy the comedy on how dumb this vendor is and how easily they got caught.

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      • pep_researcherP pep_researcher

        @ogsteve This is not right. Endotoxins residues even without active bacteria can cause serious adverse research outcomes and they don't get filtered out using regular pep filters.

        Recall that JEEP Tirz batches with horrendous endotoxins levels had passed sterility tests. You can find COAs on this forum.

        senex_musS Offline
        senex_musS Offline
        senex_mus
        wrote last edited by senex_mus
        #43

        @pep_researcher that's not what I read or agreed to.

        Essentially, filtering will remove bacteria and no more endotoxins will be introduced.

        No way to get rid of the current endotoxin load, just limit or stop more being introduced, and of course, injecting your test subject with bacteria.

        Endotoxins can be introduced anywhere in the manufacturing process. No bacteria required even. There is no way to get rid of them w/o destroying the product unfortunately. GMP is the product being filtered as it's being pumped into the fill room for lophilization. Any slight contamination problem in the fill, and the product is non-sterile. Sterility in the fill that I worked in was much, much more rigorous that any hospital operating room that I've seen.

        Filter sterilization will at least remove bacteria and limit further endotoxin production. Best you can do since there is no iron clad guarantee of sterility.

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        • pep_researcherP pep_researcher

          @ogsteve This is not right. Endotoxins residues even without active bacteria can cause serious adverse research outcomes and they don't get filtered out using regular pep filters.

          Recall that JEEP Tirz batches with horrendous endotoxins levels had passed sterility tests. You can find COAs on this forum.

          O Offline
          O Offline
          ogsteve
          wrote last edited by ogsteve
          #44

          @pep_researcher maybe re-read my comment. I never said anything about filtering endotoxins. 😉

          Endo bad…
          Sterilty… dont care. Just filter.

          The Endo that’s in the vial doesn’t get any worse while it’s in the vial.

          The bacteria that cause the endo get filtered at recon.

          S 1 Reply Last reply
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          • O ogsteve

            @pep_researcher maybe re-read my comment. I never said anything about filtering endotoxins. 😉

            Endo bad…
            Sterilty… dont care. Just filter.

            The Endo that’s in the vial doesn’t get any worse while it’s in the vial.

            The bacteria that cause the endo get filtered at recon.

            S Offline
            S Offline
            Stevepep
            wrote last edited by Stevepep
            #45

            @ogsteve the basic fact you are missing is if there is a sterility issue there are endotoxins they go hand and hand and most labs suck at testing for them where sterility is fairly easy to test for.

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