SS-31 (Elamipretide) — Research Dosing Protocol

[ResearchCat]

I just finished my SS-31 run. I decided to do phases 1-2 and skip the burst phase mainly due to costs. I did a concentration of 10mg in 40 units (~24mg/ml). I occasional mild ISR but no worse than from 12 units of glow. I found using the gansulin pen made it a breeze.

Now running MOTS-C and planning to add NAD+.

[MyB]

@researchcat Dr Sawicki dropped a short on YouTube about how antioxidants block Mot-C from entering the cell. So you’ll probably want to research Mot and NAD on different runs.
https://youtube.com/shorts/W2a9qZ7vP1k?si=3-iRJFN2so7hVyLx

[ResearchCat]

@MyB that’s interesting. My inclination was to do them all separately, but it seems a lot of people (YouTubers) are suggesting you blast everything all together. Appreciate the tip.

Edit: Oh, I see, so not saying you can’t research them together, but that you shouldn’t research them at the same time of day.

[MyB]

@researchcat i made a post under the mot-c dosing topic. https://community.peptidecritic.com/post/2819
Based on half life’s, dosing Mot-C then dose NAD+ 8 hrs later would get full benefits of both without overlap.

[MacLeeezy]

This post is deleted!

[Katya]

@diegoc newbie here.

How lengthy is the Fox04 protocol?
Would a fasting protocol work similarly to Fox04?

TIA

[diegoc]

I would think fasting works on Autophogy and reset, and Fox04 clears scenesant cells.

Many different protocols for length of time

[PeptidePete]

said in SS-31 (Elamipretide) — Research Dosing Protocol:

@Randy said in SS-31 (Elamipretide) — Research Dosing Protocol:

@PeptidePete let us know how it goes. I just finished it a few days ago. My only real complaint is the itchy ISR i got as the dose went up.

Currently finishing up some 40mg vials which I was reconstituting with <1ml and it was too uncomfortable at that concentration in one pin in one spot. Switched to doubling the dilution, two 1ml pins in adjacent sites. Everything hunky-dory now.

Also was pinning in the AM, now switched to PM as my pin load is less at night. Less chance of cross-talk issues, and perhaps its magic works better while the body is doing other clean up. Who knows.

Finished the 50mg daily blast over 2+ months. Can't say I feel any different but then again I felt pretty damn good to start with and was 95% optimized. Other than mild ISR no sides. Should have some flipping good mitochondria now...lol. PS- as previously noted, I would suggest titration over some period of time and not just starting up 50mg from the get go. To each his own.

Still running MOTS/NAD+ because, well, I love the NAD and like the MOTS.

Bonus tip for ultimate mito health maintenence- fish eggs (roe), and if your finances allow, specifically sturgeon roe. Bought in 500g or 1kg tins, it is not that much more of an investment compared to some peptides. Your research on incorporation into cellular membranes will blow your mind. Explains and dovetails nicely with man's spread across the earth via coastal/ocean routes.

[justalittleprick]

I've seen SS-31 recommendations ranging from 2-4mg daily up to 40mg daily. It sounds like the 40mg comes from a trial for people with Barth syndrome, which is really rare and an extreme case of mitochondrial disfuntion.

For those of you researching SS-31 in the 2-4mg daily range along with or as a precursor to MOTS-C, were you happy with the effects or do you think higher doses are required?

[ResearchCat]

@PeptidePete Yeah, I don’t know that you are necessarily supposed to feel something from the SS-31 if you don’t have issues, but it should repair things that aren’t working and lay the groundwork for the MOTS-C and NAD+. I started MOTS-C a couple weeks ago and feel some more energy and no side effects. Added NAD+ this week and that has a kick that is almost scary(started at 50mg).

It is tough to tell the difference day over day without journaling, especially if you are measuring how good you feel. From pictures, measures, records, and third party opinion, I am much improved from last year, when i started out. Feel great, less pain, lift heavier, look better, people say I look great, scans say I have less fat. I need to work on inflammation, cardiac indicators, and improving muscle mass, but it is all working. I look and feel better than when i was 20 years younger.

Further along those lines, if you believe some of the research on NAD+ and cardiac health, then running the SS/MOTS/NAD protocol could have meaningful benefits for heart and overall health that might not be easy to measure without frequent, expensive testing. I guess if I live to be 100 and still have mobility and a few marbles, it worked, and if I don’t, well, I’ve spent money on dumber things.

[ResearchCat]

@justalittleprick I think of SS-31 as more laying the groundwork than building the structure. You might try one of the protocols that used SS-31, MOTS-C, and NAD+ for more immediate effect. I didn’t necessarily feel any different on SS-31, the same as I didn’t feel anything from epitalon, but that doesn’t mean it isn’t working. I am also researching other things, so it’s hard to know with certainty what is doing what if you are getting the desired results.

My opinion is that a lower dose is probably fine for baseline repair, but that depends on your age, fitness, etc. If you’re 20-something, you probably don’t need any of this stuff. If you’re 60-something, you’ll probably be amazed what the mitochondrial reset will do for your energy levels.

[diegoc]

I agree. Im in the 10mg daily phase of the ss-31 protocol.

I dont feel anything but like you said, its repairing the mitochondria.

I do know when i add the Mots-c i will feel the difference.

[justalittleprick]

@researchcat My game plan is to take ATX-0304, then layer in SS-31, and then transition to MOTS-C. I'll add NAD+ at some point.

My curiousity was around the effective dosage for SS-31.

I'm 48. I'm looking at SS-31 like an oil change for my mitochondria, not an engine overhaul. I've seen claims that 40mg daily of SS-31 has the same effect as 4mg daily for most people.

Here is a video recommending 5-10mg/day.
https://www.youtube.com/watch?v=cjUm5gkSwRk

Here is an article recommending 5mg/day.
https://www.innerbody.com/ss-31-peptide

If that is the case, that is a huge cost variance from the protocol Randy posted above - Roughly 300mg vs 1000mg over a 60 day period.

I was curious if people felt like sticking with the 4-5mg dose for 60 days was effective, based on their research.

[ResearchCat]

As mentioned above, I just completed Phase 1 and 2 of the protocol Randy recommended (~300mg total). It’s hard to say with certainty how effective it is given my goal was to seek long term benefits that are not easily measurable, and that I am researching MOTS-C and NAD+ now.

I’d suggest trying the lower dose protocol and see if you think it benefits you before ramping up to a higher dose. You can always accelerate research if you think it may benefit you more.

Anecdotally, a friend is currently researching the combination SS-31/MOTS-C/NAD+ protocol and aside from getting some tiredness after higher doses, his rat feels amazing.

[PeptidePete]

Reminded by the @researchcat that I as well experienced tiredness/energy drained feeling upon ramping up dose/frequency of SS31 which subsided and indeed reversed over a few weeks. A feature not a bug methinks.

[ResearchCat]

This post is deleted!

[ResearchCat]

I was generally researching before bed, so I didn’t really notice.

[beachfun]

I started the SS 31 and would feel a crash at 4, tried MotsC then crashed again, NAD+ did nothing for me either. This was the low and slow phase while finishing 6 weeks 5 amino 1mq. So tried the Lipo-C with glutathione and WOW!!! All day energy and no crash into the 2nd day. I bought a kit and started 3rd week of Lipo-C with 75 units IM 2xs a week with a great mood too. I will try the 5mg MotsC but was only at 2.5mg but thought Id share my great experience with LipoC and will do SS31 later. Im 62 and working hard on a big kitchen remodel job solo, so I need to feel good to perform well working hard physically.

[Stan Douglas]

Just putting this out there for anyone else with 10x 30mg vials that may want to adopt a similar RandyRat Cycle on their critter:

Phase 1: .4mg x15 days, first 2 vials 1.5mL 20u a draw

Phase 2: 10mg x 12 days, vials 3-6 .6mL 20u draw

Phase 3: 30mg every third day, last 4 vials .5mL 50u draw

I'm starting tomorrow, suggestions are welcome.

[Eleanor]

@Stan-Douglas Please keep us posted. No way I would attempt the heavy protocol on my kitty, but the one you posted sounds fairly reasonable. Please confirm your Phase 1 as .4mg? Much thanks and I wish you great success.