How much peptide is lost during filtering?

[chrams]

And as a follow up - im not talking about if any total mass is lost, I understand if you don't flush the filter with bac water, a certain amount will be "trapped" in there. I'm talking about how much the dilution gets lowered- ie how much mg per ml

[Randy]

The AI is mostly wrong. The amount of loss (sticking to the filter) Isnt measurable. You arent losing enough mass (mg/ml) for it to matter.

we cover how much liquid is lost here https://www.youtube.com/watch?v=0EHHXTdlYCw

[chrams]

@randy Thanks for the reply! And that's kind of what I assumed. Just curious, how did you come to that conclusion? Is this just something you learned by talking to lab workers / researchers?

[Randy]

A lab owner I'm friends with who's much smarter than I am did the testing to debunk any of that.

[Reactive3078]

@Randy can we get this back up on randytube?

[Stan Douglas]

Oops, you provided a link to YT and not the Rumble Channel @Randy

[Mydogdaryl423]

@stan-douglas That was a post from March.

[SkepticJen]

So I was doing the additional BAC at the end like on our Randy-tube. Then I watched Warrior something video and she just sent air through and it forced
almost all the liquid trapped in the filter. That made way more sense to me and I just did it yesterday and it worked.

[Stevepep]

@SkepticJen that’s what I do as well. @randy has also suggested this as well

[ResearchCat]

@SkepticJen said:

So I was doing the additional BAC at the end like on our Randy-tube. Then I watched Warrior something video and she just sent air through and it forced
almost all the liquid trapped in the filter. That made way more sense to me and I just did it yesterday and it worked.

I have found - I don’t know if this is by design - that when trying to draw air back to push through the filter, it doesn’t work. At least not with the 13mm filters from Randy. Might just be me, but I have had little luck clearing the filter with air.

[vpeps]

@ResearchCat I just loosen the filter's connection with the barrel slightly, then I pull the plunger back, re-secure the connection and push the air.

[denti]

Doing an air purge you still don’t get all the holdback. But still better than nothing. I stop doing a Bac flush back and just do the air purge as well. Less hassle and saves time.

[Stan Douglas]

@Mydogdaryl423

Hmmm, it was put in my New Topic link for some reason.
MY BAD! 🫣

[Mydogdaryl423]

@stan-douglas Yes someone had added a new comment. The video link from Randy was from March. No worries.

[SkepticJen]

@ResearchCat Yes - unscrew the syringe and pull plunger, then reattach to filter. Seemed to get most of it.

[ogsteve]

My flush/rinse depends on what I’m filtering! If it’s bpc157 I’m not worried about if it’s eloralintide or even ss-31 I’m running an extra 5-10iu thru.