Do you actually need a vent needle when filtering into a 5mL sterile vial?
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@myb this! The primary context I see vent needles being used is for loading pen cartridges, where they are pressure neutral and filling the Cartridge without a vent may (will?) push the plug out. When I am moving peps from one vial to another, I do not vent.
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@myb this! The primary context I see vent needles being used is for loading pen cartridges, where they are pressure neutral and filling the Cartridge without a vent may (will?) push the plug out. When I am moving peps from one vial to another, I do not vent.
@ResearchCat
Ahhh - can in fact confirm that attempting to fill a pen cartridge with 2.5ml of BAC & 30mg of RETA without a vent needle will in fact eject the plug like a pop gun and spray the liquid all over one’s own fat guts.
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@statieeight
laughing with you, not at you. I haven’t had that happen, but I have blown over the vent and spilled SS-31 all over my hands and desk.I did not expect reconstituted peptides to be so, well, sticky. what a mess!
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@ResearchCat
Ahhh - can in fact confirm that attempting to fill a pen cartridge with 2.5ml of BAC & 30mg of RETA without a vent needle will in fact eject the plug like a pop gun and spray the liquid all over one’s own fat guts.@STATIEEIGHT said in Do you actually need a vent needle when filtering into a 5mL sterile vial?:
@ResearchCat
Ahhh - can in fact confirm that attempting to fill a pen cartridge with 2.5ml of BAC & 30mg of RETA without a vent needle will in fact eject the plug like a pop gun and spray the liquid all over one’s own fat guts.Lol. I sacrificed a cartridge for my first time filling one using tap water so I can practice. Good thing I did. Totally forgot the vent needle and sure enough it popped out.
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This post is deleted!
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I am wondering if anyone has had an issue with the breather needle leaking peptide while filtering? Everything was going fine until the last .5ml. I was pushing in slowly like usual, but all of the sudden peptide bubbled up out of the breather needle.
@kmckinley
You’ve got to keep the tip of the breather needle above the level of the liquid. -
Yeah, I have had an overflow before. Part of the learning process. Also note that depending on how many vials you are trying to combine into one cart, you might end up with more liquid than will fit in the cart, no matter how convinced you are that you can make it happen.
(looks in the mirror in NAD+) -
@kmckinley
You’ve got to keep the tip of the breather needle above the level of the liquid.@STATIEEIGHT This was the issue I believe! Ok I need to keep a closer eye on the level I was moving 3ml to 3ml and thought I had it perfect. Thank you!
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Yeah, I have had an overflow before. Part of the learning process. Also note that depending on how many vials you are trying to combine into one cart, you might end up with more liquid than will fit in the cart, no matter how convinced you are that you can make it happen.
(looks in the mirror in NAD+)@ResearchCat Thank you! I was moving 3ml to 3ml so it was supposed to go smoothly haha. I will pay closer attention to how far the breather is in next time for sure!
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