Do you actually need a vent needle when filtering into a 5mL sterile vial?
-
Been going back and forth on this for weeks and want to hear from people who actually filter.
My setup: reconstitute in the original vial (0.5-2mL bac water depending on the peptide), draw the full contents into a 3mL Luer Lock syringe, attach a 13mm PES 0.22um filter, attach a fresh 21G on the output side, push through into a sealed 5mL sterile receiving vial (ALK).
My biggest transfer is about 2.15mL into a 5mL vial. That is less than half the vial's air space.
I keep seeing people say you need a vent needle in the receiving vial to let displaced air escape while you push liquid in. Some people use a plain 18G, some say you need a filtered 0.2um hydrophobic vent, and those things are either impossible to source as an individual or cost $300+ each.
But if I am only pushing ~2mL into a 5mL vial, is the back-pressure actually a problem? It seems like the air just compresses a bit and the syringe plunger handles it fine. Has anyone here actually tried filtering into a sealed 5mL vial without any vent at all?
If you do use a vent, are you using a plain open needle or a filtered one? And if plain, does that not defeat the entire purpose of filtering since you just gave room air a direct path into the vial you are trying to keep clean?
Genuinely trying to figure out the right move here.Appreciate any real experience.
-
My experience. Your empty vial should be under vacuum, from being sterilized, and will pull your liquid in. If you hit a stopping point pull back on syringe and finish your push. Repeat pull and push as needed.
I only vent on pen vials. Adding a filter to your vent is a personal choice, some do some don’t. You filter the peptide because sterility is often unknown. -
@myb this! The primary context I see vent needles being used is for loading pen cartridges, where they are pressure neutral and filling the Cartridge without a vent may (will?) push the plug out. When I am moving peps from one vial to another, I do not vent.
-
@myb this! The primary context I see vent needles being used is for loading pen cartridges, where they are pressure neutral and filling the Cartridge without a vent may (will?) push the plug out. When I am moving peps from one vial to another, I do not vent.
@ResearchCat
Ahhh - can in fact confirm that attempting to fill a pen cartridge with 2.5ml of BAC & 30mg of RETA without a vent needle will in fact eject the plug like a pop gun and spray the liquid all over one’s own fat guts.
-
@statieeight
laughing with you, not at you. I haven’t had that happen, but I have blown over the vent and spilled SS-31 all over my hands and desk.I did not expect reconstituted peptides to be so, well, sticky. what a mess!
-
@ResearchCat
Ahhh - can in fact confirm that attempting to fill a pen cartridge with 2.5ml of BAC & 30mg of RETA without a vent needle will in fact eject the plug like a pop gun and spray the liquid all over one’s own fat guts.@STATIEEIGHT said in Do you actually need a vent needle when filtering into a 5mL sterile vial?:
@ResearchCat
Ahhh - can in fact confirm that attempting to fill a pen cartridge with 2.5ml of BAC & 30mg of RETA without a vent needle will in fact eject the plug like a pop gun and spray the liquid all over one’s own fat guts.Lol. I sacrificed a cartridge for my first time filling one using tap water so I can practice. Good thing I did. Totally forgot the vent needle and sure enough it popped out.
-
This post is deleted!
