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  1. Randy the Rats Research Forum
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  3. NAD+ Reconstitution advice needed
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NAD+ Reconstitution advice needed

Scheduled Pinned Locked Moved Peptide Discussion
mixingbeginner-questionnad
21 Posts 7 Posters 313 Views
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  • B Offline
    B Offline
    brandenscheidecker
    wrote on last edited by
    #1

    found some NAD+ on gray, 100Mg vials, it came in 10ML Vial. idk why lol adding 1.5 ML of back water gives my Rat a 20mg dose @ 30 units
    any reason I should add more to this? Less sting? hate to push more in the syringe than needed.

    J EXTROPIANE gym.ratG 3 Replies Last reply
    1
    • S Offline
      S Offline
      sfc94
      wrote on last edited by
      #2

      @randy somewhat unrelated question here but I just reconstituted some NAD from Nexaph and it didn’t seem to have a vacuum in the vial when reconstituting (had to actually push the BAC through rather than preventing it from coming out quickly) - should I be worried?

      1 Reply Last reply
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      • B brandenscheidecker

        found some NAD+ on gray, 100Mg vials, it came in 10ML Vial. idk why lol adding 1.5 ML of back water gives my Rat a 20mg dose @ 30 units
        any reason I should add more to this? Less sting? hate to push more in the syringe than needed.

        J Offline
        J Offline
        JtCjEfF
        wrote last edited by
        #3

        @brandenscheidecker Curious how this went for you. Ive read that a 1000mg vial should be reconstituted with 10ml bac water to reduce sting. I have some coming from crush, but the site says its in 3ml vials so Im not quite sure how the heck I'm going to recon it yet.

        N 1 Reply Last reply
        0
        • B brandenscheidecker

          found some NAD+ on gray, 100Mg vials, it came in 10ML Vial. idk why lol adding 1.5 ML of back water gives my Rat a 20mg dose @ 30 units
          any reason I should add more to this? Less sting? hate to push more in the syringe than needed.

          EXTROPIANE Offline
          EXTROPIANE Offline
          EXTROPIAN
          wrote last edited by EXTROPIAN
          #4

          @brandenscheidecker I have a kit of 100mg NAD+ I been reconstituting w 1ml. I haven't had any issues with stinging other than just pinning. I'm now seeing NAD+ buffed which includes pH-stabilizing agents to prevent degradation. Im going to purchase a NAD Buffed to see the difference before i purchase a whole set to buff what I already have.

          gym.ratG 1 Reply Last reply
          0
          • J JtCjEfF

            @brandenscheidecker Curious how this went for you. Ive read that a 1000mg vial should be reconstituted with 10ml bac water to reduce sting. I have some coming from crush, but the site says its in 3ml vials so Im not quite sure how the heck I'm going to recon it yet.

            N Online
            N Online
            Nuncles
            wrote last edited by
            #5

            @JtCjEfF said:

            @brandenscheidecker Curious how this went for you. Ive read that a 1000mg vial should be reconstituted with 10ml bac water to reduce sting. I have some coming from crush, but the site says its in 3ml vials so Im not quite sure how the heck I'm going to recon it yet.

            I recently got some 1000mg from Crush and it came in a 10mL vial. Slight sting if pushed too fast. But I've only had Hallandale compounded NAD to compare.

            1 Reply Last reply
            0
            • B Offline
              B Offline
              brandenscheidecker
              wrote last edited by
              #6

              I used the entire kit! I will stay away from 100mg kits as Im taking 50mg daily It had a slight sting but was not to bad, I did find that I needed to used 1.5mg of bac due to the larger puck size in the vial, using 1ml didnt seem to dissolve it. Since then I have purchased a 500mg buffered kit from nexaph and it has almost no sting, I have some 1000mg kits coming from china.

              E gym.ratG 2 Replies Last reply
              0
              • B brandenscheidecker

                I used the entire kit! I will stay away from 100mg kits as Im taking 50mg daily It had a slight sting but was not to bad, I did find that I needed to used 1.5mg of bac due to the larger puck size in the vial, using 1ml didnt seem to dissolve it. Since then I have purchased a 500mg buffered kit from nexaph and it has almost no sting, I have some 1000mg kits coming from china.

                E Offline
                E Offline
                Eleanor
                wrote last edited by Eleanor
                #7

                @brandenscheidecker said:

                I used the entire kit! I will stay away from 100mg kits as Im taking 50mg daily It had a slight sting but was not to bad, I did find that I needed to used 1.5mg of bac due to the larger puck size in the vial, using 1ml didnt seem to dissolve it. Since then I have purchased a 500mg buffered kit from nexaph and it has almost no sting, I have some 1000mg kits coming from china.

                I can't do without NAD+ buf; will give up all others to keep it. S1research has 1000mg NAD+ buf if you want some while waiting on your shipment.

                s1research.net

                1 Reply Last reply
                0
                • B brandenscheidecker

                  found some NAD+ on gray, 100Mg vials, it came in 10ML Vial. idk why lol adding 1.5 ML of back water gives my Rat a 20mg dose @ 30 units
                  any reason I should add more to this? Less sting? hate to push more in the syringe than needed.

                  gym.ratG Offline
                  gym.ratG Offline
                  gym.rat
                  wrote last edited by
                  #8

                  @brandenscheidecker 100mg vial is kind of a waste if you ask me. That's like getting all of 2 doses. Starter is 25mg and most people run 50mg with some maxing at 100mg a dose.

                  But yes more BAC should help with the sting b/c NAD+ is very acidic.

                  Data collector for https://peptideprotocols.app/

                  1 Reply Last reply
                  0
                  • EXTROPIANE EXTROPIAN

                    @brandenscheidecker I have a kit of 100mg NAD+ I been reconstituting w 1ml. I haven't had any issues with stinging other than just pinning. I'm now seeing NAD+ buffed which includes pH-stabilizing agents to prevent degradation. Im going to purchase a NAD Buffed to see the difference before i purchase a whole set to buff what I already have.

                    gym.ratG Offline
                    gym.ratG Offline
                    gym.rat
                    wrote last edited by
                    #9

                    @martinmarin My in home female lab rat started on unbuffered and I moved her over to buffered. Pretty big difference in pain. Plus the buffered is a bit stronger and it lasts longer. NAD+ is one of the peps that degrades rapidly, so once you recon it you better get using it.

                    Data collector for https://peptideprotocols.app/

                    EXTROPIANE 1 Reply Last reply
                    0
                    • B brandenscheidecker

                      I used the entire kit! I will stay away from 100mg kits as Im taking 50mg daily It had a slight sting but was not to bad, I did find that I needed to used 1.5mg of bac due to the larger puck size in the vial, using 1ml didnt seem to dissolve it. Since then I have purchased a 500mg buffered kit from nexaph and it has almost no sting, I have some 1000mg kits coming from china.

                      gym.ratG Offline
                      gym.ratG Offline
                      gym.rat
                      wrote last edited by
                      #10

                      @brandenscheidecker NAD+ with zero sting honestly makes me think bad product. You can't convince me otherwise.

                      Data collector for https://peptideprotocols.app/

                      E 1 Reply Last reply
                      0
                      • gym.ratG gym.rat

                        @brandenscheidecker NAD+ with zero sting honestly makes me think bad product. You can't convince me otherwise.

                        E Offline
                        E Offline
                        Eleanor
                        wrote last edited by
                        #11

                        @gym.rat said:

                        @brandenscheidecker NAD+ with zero sting honestly makes me think bad product. You can't convince me otherwise.

                        I can guarantee you, that is NOT true! Either that, or I have the biggest placebo response in history! It's okay if you're not convinced. Knock yourself out. Sting baby, sting. 😨

                        1 Reply Last reply
                        1
                        • J Offline
                          J Offline
                          JtCjEfF
                          wrote last edited by
                          #12

                          OK, good to know that the buffered helps with the sting that much. I have a kit of 1000mg buffered coming from Crush, so I will plan to recon that at a normal amount for dose/units then. Are y'all using normal bac water, or the sodium chloride version?

                          E gym.ratG 2 Replies Last reply
                          1
                          • J JtCjEfF

                            OK, good to know that the buffered helps with the sting that much. I have a kit of 1000mg buffered coming from Crush, so I will plan to recon that at a normal amount for dose/units then. Are y'all using normal bac water, or the sodium chloride version?

                            E Offline
                            E Offline
                            Eleanor
                            wrote last edited by
                            #13

                            @JtCjEfF I always use more BAC than needed, regardless of what the pep is. I use Randy's recon tool and will get it to as close to a number ending in '5' as possible; generally between 20 and 60 units (for syringe). If you're using a pin, IDK what the numbers are.

                            J 1 Reply Last reply
                            0
                            • E Eleanor

                              @JtCjEfF I always use more BAC than needed, regardless of what the pep is. I use Randy's recon tool and will get it to as close to a number ending in '5' as possible; generally between 20 and 60 units (for syringe). If you're using a pin, IDK what the numbers are.

                              J Offline
                              J Offline
                              JtCjEfF
                              wrote last edited by
                              #14

                              @Eleanor ok thanks, Ill give that "a shot". 🥁🥁

                              1 Reply Last reply
                              1
                              • J JtCjEfF

                                OK, good to know that the buffered helps with the sting that much. I have a kit of 1000mg buffered coming from Crush, so I will plan to recon that at a normal amount for dose/units then. Are y'all using normal bac water, or the sodium chloride version?

                                gym.ratG Offline
                                gym.ratG Offline
                                gym.rat
                                wrote last edited by
                                #15

                                @JtCjEfF Regular BAC water, just dilute your vial. I've done 2ml and 3ml for 500mg vials. I think I like 3ml better.

                                Data collector for https://peptideprotocols.app/

                                E 1 Reply Last reply
                                0
                                • gym.ratG gym.rat

                                  @JtCjEfF Regular BAC water, just dilute your vial. I've done 2ml and 3ml for 500mg vials. I think I like 3ml better.

                                  E Offline
                                  E Offline
                                  Eleanor
                                  wrote last edited by
                                  #16

                                  @gym.rat said:

                                  @JtCjEfF Regular BAC water, just dilute your vial. I've done 2ml and 3ml for 500mg vials. I think I like 3ml better.

                                  I'll BAC as many as 5ml because of the higher unit, the syringe pulls/pushes better, IMO.

                                  E 1 Reply Last reply
                                  0
                                  • N Online
                                    N Online
                                    Nuncles
                                    wrote last edited by
                                    #17

                                    Is that why I see a lot of places give the option for 500mg and 1000mg? Is the 500 supposed to sting less? And what size vials do 500mg normally come in?

                                    E 1 Reply Last reply
                                    0
                                    • gym.ratG gym.rat

                                      @martinmarin My in home female lab rat started on unbuffered and I moved her over to buffered. Pretty big difference in pain. Plus the buffered is a bit stronger and it lasts longer. NAD+ is one of the peps that degrades rapidly, so once you recon it you better get using it.

                                      EXTROPIANE Offline
                                      EXTROPIANE Offline
                                      EXTROPIAN
                                      wrote last edited by
                                      #18

                                      @gym.rat Yes i might just blow through them. Up the dose tongauge the difference in this case. They are 100mg vials. But I am in my sweet spot though. Well my ph test strips are in my amazon cart now. Man this is getting expensive. lol

                                      E 1 Reply Last reply
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                                      • N Nuncles

                                        Is that why I see a lot of places give the option for 500mg and 1000mg? Is the 500 supposed to sting less? And what size vials do 500mg normally come in?

                                        E Offline
                                        E Offline
                                        Eleanor
                                        wrote last edited by
                                        #19

                                        @Nuncles said:

                                        Is that why I see a lot of places give the option for 500mg and 1000mg? Is the 500 supposed to sting less? And what size vials do 500mg normally come in?

                                        I'm not sure, Nuncles; it's just the amount in the vial; the amount of BAC is still a personal choice. In 500mg I use 5ml BAC, which makes it a 30 unit pull in syringe for 30mg dose. In 1000mg I use up to 10ml for the same 30 dose/units. I like my numbers to line up as the same; saves confusion.

                                        Whether it's BUFFERED is what matters as far as tolerability (sting).

                                        1 Reply Last reply
                                        1
                                        • EXTROPIANE EXTROPIAN

                                          @gym.rat Yes i might just blow through them. Up the dose tongauge the difference in this case. They are 100mg vials. But I am in my sweet spot though. Well my ph test strips are in my amazon cart now. Man this is getting expensive. lol

                                          E Offline
                                          E Offline
                                          Eleanor
                                          wrote last edited by
                                          #20

                                          @martinmarin said:

                                          Man this is getting expensive. lol

                                          YA THINK?! LOL! There are true professional pep hogs here... I'm an amateur.

                                          1 Reply Last reply
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