Bacteriostatic Water
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This has probably been asked before, but I want to make sure for my situation. I know bac water is usually good for about 2–3 weeks after it’s been punctured. My question is, can I stock up on a few vials and keep them in the fridge, and if so, for how long?
I only use about 1ml every month or two, so I wouldn’t be using the same bac water vial for my next reta vial. And it's pretty hard as it is to order Hospira bac water.
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Also, where can I find Hospira bac water that's less than 30ml? Can't find it anywhere.
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Hospira to my knowledge only comes in 30ml You can stock up on it, unopened has an exp of 9/2027 I believe right now. Store in a cool dark place. Once opened, discard after 28 days per Hospira guidelines. Some folks keep it longer, but I try to stick to their guidelines.
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Here's a link to a study that Randy did with a lab: https://community.peptidecritic.com/post/4657
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Hospira to my knowledge only comes in 30ml You can stock up on it, unopened has an exp of 9/2027 I believe right now. Store in a cool dark place. Once opened, discard after 28 days per Hospira guidelines. Some folks keep it longer, but I try to stick to their guidelines.
@WisGal64 Thank you! May I ask where can I find Hospira bac water? I bought a vial a couple of weeks ago for $25
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@ragnatlothbrok Ouch, PeptideTest.com has it for $15 a bottle. Prices have definitely been on the rise. My first purchase, they were $10, then went to 11, now 15. Peptide test didn't jump as high as others have though
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@ragnatlothbrok Ouch, PeptideTest.com has it for $15 a bottle. Prices have definitely been on the rise. My first purchase, they were $10, then went to 11, now 15. Peptide test didn't jump as high as others have though
@WisGal64 thanks a lot! I’ll order it from here
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I just purchased 5 bottles from Peptide Test today, $15 each! I went ahead and bought 100 insulin needles, too, just in case. Can't hurt to put them away and save them.
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It's too big a bottle for my use but thought I'd put this here for anyone else in the market
30mL Hospira individually $18.95+shipping:
https://www.farrislabs.com/blogs/information/the-uses-of-bacteriostatic-water-and-where-to-get-it -
Some researchers go much longer with Hospira than 28 day rule.
It's about personal judgement and risk tolerance. But if Peter of Janoshik is to be quoted, the BAC water and 28 day rule is over hyped. He said there's only sterile water available in Europe. BAC is more of north American thing. They use sterile water vial for long long time without any recon issues.
Disclaimer: NOT an advice, just quoting for the context.
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@pep_researcher I did see that video. I started going down the rabbit hole of Sterile vs Benzyl, there is a ton of info out there based on the chemistry, different medical opinions, real world experience etc. Made my head spin lol
Some interesting notes, yet to be verified, this is what I found doing different searches and using AI.
Most small, linear peptides remain chemically stable for ~1–2 weeks refrigerated in sterile water.
This is because:
They lack complex tertiary structure
They don’t rely on disulfide bonds
They resist aggregation
Hydrolysis is slow at cold temperaturesExamples of peptides that fall into this “generally stable” category (chemically speaking):
ARA‑290
Thymosin Beta‑4 fragments
GHK‑type tripeptides
Short melanocortin fragments
Short metabolic peptides (5–15 aa)These peptides degrade primarily through:
Hydrolysis (slow in cold water)
Oxidation (minimized if oxygen exposure is low)So their stability is driven by intrinsic chemistry, not the presence or absence of benzyl alcohol
The information AI used came from(not from any specific resources):
Biochemical mechanisms
Pharmaceutical formulation science
Regulatory principles
Peptide stability patterns
General scientific knowledgeDefinitely leads to more questions
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I have 10 vials of 2MG Bac Water on the way from Advanced Research Pep. They're not a company listed on this site yet but I have used them a couple times prior to finding this site.
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@stan-douglas do you test the PH before reconstituting?
I like the idea of offering different sizes for sure, but I don't see a CoA and if the PH balance isn't right, it could ruin the pep. Have you used their bac water in the past?
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@pep_researcher I did see that video. I started going down the rabbit hole of Sterile vs Benzyl, there is a ton of info out there based on the chemistry, different medical opinions, real world experience etc. Made my head spin lol
Some interesting notes, yet to be verified, this is what I found doing different searches and using AI.
Most small, linear peptides remain chemically stable for ~1–2 weeks refrigerated in sterile water.
This is because:
They lack complex tertiary structure
They don’t rely on disulfide bonds
They resist aggregation
Hydrolysis is slow at cold temperaturesExamples of peptides that fall into this “generally stable” category (chemically speaking):
ARA‑290
Thymosin Beta‑4 fragments
GHK‑type tripeptides
Short melanocortin fragments
Short metabolic peptides (5–15 aa)These peptides degrade primarily through:
Hydrolysis (slow in cold water)
Oxidation (minimized if oxygen exposure is low)So their stability is driven by intrinsic chemistry, not the presence or absence of benzyl alcohol
The information AI used came from(not from any specific resources):
Biochemical mechanisms
Pharmaceutical formulation science
Regulatory principles
Peptide stability patterns
General scientific knowledgeDefinitely leads to more questions
@WisGal64 Wow that's some long deep rabbit hole! LOL
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Some researchers go much longer with Hospira than 28 day rule.
It's about personal judgement and risk tolerance. But if Peter of Janoshik is to be quoted, the BAC water and 28 day rule is over hyped. He said there's only sterile water available in Europe. BAC is more of north American thing. They use sterile water vial for long long time without any recon issues.
Disclaimer: NOT an advice, just quoting for the context.
@pep_researcher said in Bacteriostatic Water:
Some researchers go much longer with Hospira than 28 day rule.
It's about personal judgement and risk tolerance. But if Peter of Janoshik is to be quoted, the BAC water and 28 day rule is over hyped. He said there's only sterile water available in Europe. BAC is more of north American thing. They use sterile water vial for long long time without any recon issues.
Disclaimer: NOT an advice, just quoting for the context.
That's Hospira covering their butt. I am comfortable using Bac water, especially a high grade reputable one like Hospira, definitely well past 30 days. Fridge it after it has been opened. We've got plenty of research and anecdotal data to show they're good for up to 90 days.
But remember, that's 90 days TOTAL since opened. Doesn't mean a half bottle can sit in your fridge for 89 days, you use it to recon, then its magically good for another 30 days while you research with whatever peptide you recon with.
I would NOT go past 90 days, the research shows the bac water sterility breaks down fast after that. So not worth it just to save whatever small amount. I am not worried about potency loss, and neither should you. I am worried about bacterial growth.
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@stan-douglas do you test the PH before reconstituting?
I like the idea of offering different sizes for sure, but I don't see a CoA and if the PH balance isn't right, it could ruin the pep. Have you used their bac water in the past?
@WisGal64
I have not used their water in the past nor have I ever thought of testing the pH from any other brands.
Spose I should start... I haven't run into a problem though. -
Is there any better version of BAC available? think what they put in the mounjaro kwikpen.
Buffered bacteriostatic saline / preserved PBS (Benzyl alcohol + NaCl + phosphate buffer)
@marco1 Mounjaro or Zepbound single dose pens or vials have no preservatives. That's why they are strongly advised to not split like so many YouTube videos demo to save cost or custom dosing. People sometimes do it anyway and add Hospira BAC water to extracted fat juice.
The multidose KwikPens or multidose vials (in the future) would have preservative like BAC water.
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@pep_researcher said in Bacteriostatic Water:
Some researchers go much longer with Hospira than 28 day rule.
It's about personal judgement and risk tolerance. But if Peter of Janoshik is to be quoted, the BAC water and 28 day rule is over hyped. He said there's only sterile water available in Europe. BAC is more of north American thing. They use sterile water vial for long long time without any recon issues.
Disclaimer: NOT an advice, just quoting for the context.
That's Hospira covering their butt. I am comfortable using Bac water, especially a high grade reputable one like Hospira, definitely well past 30 days. Fridge it after it has been opened. We've got plenty of research and anecdotal data to show they're good for up to 90 days.
But remember, that's 90 days TOTAL since opened. Doesn't mean a half bottle can sit in your fridge for 89 days, you use it to recon, then its magically good for another 30 days while you research with whatever peptide you recon with.
I would NOT go past 90 days, the research shows the bac water sterility breaks down fast after that. So not worth it just to save whatever small amount. I am not worried about potency loss, and neither should you. I am worried about bacterial growth.
@Neil-McCauley said in Bacteriostatic Water:
@pep_researcher said in Bacteriostatic Water:
Some researchers go much longer with Hospira than 28 day rule.
It's about personal judgement and risk tolerance. But if Peter of Janoshik is to be quoted, the BAC water and 28 day rule is over hyped. He said there's only sterile water available in Europe. BAC is more of north American thing. They use sterile water vial for long long time without any recon issues.
Disclaimer: NOT an advice, just quoting for the context.
That's Hospira covering their butt. I am comfortable using Bac water, especially a high grade reputable one like Hospira, definitely well past 30 days. Fridge it after it has been opened. We've got plenty of research and anecdotal data to show they're good for up to 90 days.
But remember, that's 90 days TOTAL since opened. Doesn't mean a half bottle can sit in your fridge for 89 days, you use it to recon, then its magically good for another 30 days while you research with whatever peptide you recon with.
I would NOT go past 90 days, the research shows the bac water sterility breaks down fast after that. So not worth it just to save whatever small amount. I am not worried about potency loss, and neither should you. I am worried about bacterial growth.
Thanks, I'm aware. I was pointing out that still there are biohackers who go way beyond 90 days and live.
TBH all these day rules and all seem made up based on personal judgement.
In fact someone posted an actual test of BAC water benzyl alcohol efficacy across 30, 60, 90, and 120 days on Reddit--room temp and refrigerated both. Even at 120 days there was only a slight drop in BA amount.
If I find the post, will link it here.
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@pep_researcher Randy posted Hospira test results somewhere here. At 120 days it feel off the cliff.
https://community.peptidecritic.com/post/4935
