Bacteriostatic Water vs Sterile Water
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Many people mistakenly believe that bacteriostatic water and sterile water are the same thing and that can cause dangerous results due to severe infection risk.
Sterile water is 100% distilled water while sterile saline water is distilled and includes 0.9% sodium chloride but neither has any preservatives so once opened, it is at high risk for bacterial/microbial contamination and only should only be used for immediate single use doses.
Bacteriostatic water is sterile water with 0.9% benzyl alcohol added. It actively kills bacteria and can keep the peptide mixture stable for 14-28 days after opening.
Because bac water alone can cause mild stinging/irritation for nasal solutions, it is recommended to first reconstitute the peptide with bac water to prevent bacterial growth. Then combine that mixture with sterile 0.9% saline to dilute it and prevent irritation to the sinuses.
Edit - There are also other peptides that you shouldn't reconstitute with BAC water. These peptides require acetic acid water (because they have a net positive charge at neutral pH that causes them to aggregate rather than dissolve.
Some peptides that are better reconstituted with acetic acid water include:
- GHK-Cu — the copper complex is poorly soluble in neutral water
- AOD-9604 — often requires mild acid for complete dissolution
- IGF-1 LR3 — acidic conditions prevent aggregation
- GHRP-2 and GHRP-6
- Melanotan 1 and Melanotan 2
- NAD+ - dissolves in BAC water but benefits from acidic pH for improved stability and to prevent crystallization at higher concentrations
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Randy did a test showing BA is still strong after 4 months even at room temperature AND exposed to air.
I wish he would had continued it to see how long it took to get to that .3%, where bacteriostasis stops being reliable.
Double and triple check EVERYTHING yourselves though and don't take anything I say for it's word! I don't have one of dem edumacations in da field. I used spellcheck to type THIS out and STILL had to go back and correct it!!!
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Randy did a test showing BA is still strong after 4 months even at room temperature AND exposed to air.
I wish he would had continued it to see how long it took to get to that .3%, where bacteriostasis stops being reliable.
Double and triple check EVERYTHING yourselves though and don't take anything I say for it's word! I don't have one of dem edumacations in da field. I used spellcheck to type THIS out and STILL had to go back and correct it!!!
@Stan-Douglas is the post from @randy gone? Can’t open it
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@Stan-Douglas is the post from @randy gone? Can’t open it
@RagnatLothbrok said in Bacteriostatic Water vs Sterile Water:
is the post from @randy gone? Can’t open it
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Hey guys. New here.. Has anyone heard of using BAC sodium chloride for reconstitution of their GLP1? Tirzepatide contains sodium already..
It isn't recommended to use bacteriostatic sodium chloride for most peptides. The problem is with the sodium chloride. Peptides are delicate amino acid chains and the added salt (sodium chloride) in bacteriostatic saline, even in a peptide that may contain sodium, can potentially alter its chemical stability or cause dissolve poorly (clumping) which can make it less effective.
Bacteriostatic water with 0.9% benzyl alcohol is almost always the preferred choice unless the peptide is sensitive to benzyl alcohol degradation or explicitly requires a salt-based buffer.
For your question on GLP1 analogs, there is some research and clinical compounding data that show that semaglutide, liraglutide, and exenatide do have a sensitivity to benzyl alcohol and would be more effective with sterile saline. Keep in mind though when using sterile saline that the peptide may degrade more quickly due to more preservatives.
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Is this list of peptides requiring Acetic Acid legit? I've reconstituted every peptide on that list with Bac water except AOD (which I've always heard required the AA for it to dissolve). I also know that older versions of LR3 required AA before they learned how to compound it in a way that Bac water is sufficient (the exception being if you're gonna need your large dosed reconstituted vial to last a while, then the AA gives it a better shelf life). And I can also see the logic behind high concentration NAD+ because I've experienced the crystalization on vials over 1000mg. But the Copper, GHRPs, and Melanotan 1+2?? I'm not saying it's wrong. I'm just looking for more supporting incite I guess. Because I use all of those peps quite frequently, except the AOD and secretagogues (I'm an HGH man)
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Many people mistakenly believe that bacteriostatic water and sterile water are the same thing and that can cause dangerous results due to severe infection risk.
Sterile water is 100% distilled water while sterile saline water is distilled and includes 0.9% sodium chloride but neither has any preservatives so once opened, it is at high risk for bacterial/microbial contamination and only should only be used for immediate single use doses.
Bacteriostatic water is sterile water with 0.9% benzyl alcohol added. It actively kills bacteria and can keep the peptide mixture stable for 14-28 days after opening.
Because bac water alone can cause mild stinging/irritation for nasal solutions, it is recommended to first reconstitute the peptide with bac water to prevent bacterial growth. Then combine that mixture with sterile 0.9% saline to dilute it and prevent irritation to the sinuses.
Edit - There are also other peptides that you shouldn't reconstitute with BAC water. These peptides require acetic acid water (because they have a net positive charge at neutral pH that causes them to aggregate rather than dissolve.
Some peptides that are better reconstituted with acetic acid water include:
- GHK-Cu — the copper complex is poorly soluble in neutral water
- AOD-9604 — often requires mild acid for complete dissolution
- IGF-1 LR3 — acidic conditions prevent aggregation
- GHRP-2 and GHRP-6
- Melanotan 1 and Melanotan 2
- NAD+ - dissolves in BAC water but benefits from acidic pH for improved stability and to prevent crystallization at higher concentrations
Sterile water is 100% distilled water
No. Sterile water is deionized water that's autoclaved to remove endotoxins and micro filtered to remove other contaminants. There's also close control of pH and conductivity and close monitoring of endotoxin levels.
Distilled water is meant only for external use like batteries or CPAP machines or drinking.
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