Reconstitution Compatibility chart (grabbed from another group)
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@pep_researcher the issue I guess is bacteria you have no protection if you use saline. Although I have contacts and it’s what we use to disinfect them. It’s all confusing
@Stevepep @eleanor Answered. When one says "saline BAC" it's always with 0.9% benzyl alcohol.
- Sterile water — no sodium chloride, no benzyl alcohol
- Sterile saline — sterile water, 0.9% sodium chloride, no benzyl alcohol
- Regular BAC — sterile water, 0.9% benzyl alcohol
- Saline BAC — sterile water, 0.9% sodium chloride, 0.9% benzyl alcohol.
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@pep_researcher the issue I guess is bacteria you have no protection if you use saline. Although I have contacts and it’s what we use to disinfect them. It’s all confusing
@Stevepep Further
- Sterile Water, regular BAC — hypotonic, can cause tissue stress at research sites.
- Sterile saline, saline BAC — isotonic, less tissue stress at research sites.
Having said that one cannot just use saline BAC for everything to avoid research site sting, because different peps interact differently with sodium chloride.
The posted chart is interesting although it may have some errors, like Reta + Saline BAC.
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@Stevepep Further
- Sterile Water, regular BAC — hypotonic, can cause tissue stress at research sites.
- Sterile saline, saline BAC — isotonic, less tissue stress at research sites.
Having said that one cannot just use saline BAC for everything to avoid research site sting, because different peps interact differently with sodium chloride.
The posted chart is interesting although it may have some errors, like Reta + Saline BAC.
@pep_researcher so is mots ok with bac saline and why don’t we use it for other peptides it’s far cheaper and if less irritating why not? Like maybe ghk-cu
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This might help someone.
@Snake-Pepskin
HOL' UP
You're supposed to recon IGF with 100% AA?!?!?!?
My understanding it was like... 3 drops and the rest BW. -
@pep_researcher so is mots ok with bac saline and why don’t we use it for other peptides it’s far cheaper and if less irritating why not? Like maybe ghk-cu
@pep_researcher so is mots ok with bac saline and why don’t we use it for other peptides it’s far cheaper and if less irritating why not? Like maybe ghk-cu
Go to Mot-c Reconstitution at peptide protocols (dot) app
With regard to the GHK-cu, I don't think copper will like it but I haven't checked.
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@Snake-Pepskin I have had no issues with NAD+ and just use 3ml BAC. GHK-CU same for me that one just sucks
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Yeah that chart isn't the source of truth. I wouldn't trust it.
@pep_researcher so let's correct it. What is your input/corrections?
“Knowledge is the ultimate power.
It’s not what you know that defines you, it’s that which you share, that defines you.” -
@pep_researcher so let's correct it. What is your input/corrections?
@Snake-Pepskin I don't have authoritative guide on which peps are compatible with saline BAC.
General thumb rule — most are compatible with regular BAC, some from those will be good with saline BAC too, some will not like sodium chloride and can gel.
I gave an example that I saw someone posting on Reddit that Reta didn't like saline BAC when they tried two Reta vials from the same batch. I asked an AI tool too and it said Reta will gel with saline BAC but tirzepatide and semaglutide will be fine with it.
Gist — that chart is NOT the ground truth. But I don't have the ground truth. Will do a chat session with multiple AI tools when I've extra time on hand and find out for popular peps.
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@pep_researcher so let's correct it. What is your input/corrections?
@Snake-Pepskin from my experience, AOD does not need acetic acid, been running it on my rat for months and i see no clumping and i def see results. So maybe instead of "required", the chart should say "optional" on that.
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I agree, mostly. AOD is one of those temperamental compounds that I would always want to have AA at hand. Kisspeptin is another one that is very ph sensitive.
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If the motivation of this topic is primarily to reduce stinging, I recommend a longer needle in a definite fatty area - butt cheeks. You will naturally diffuse it while driving to work. Ever since I went to 8mm Novafine (ceramic coated) needles for GHKCu, AOD, and NAD+, my girlfriend's big brown beaver reports very little stinging and no more welts. And this is simple to try.
Yes. Beavers. They can talk. -
@Eleanor Interestingly, in my first vial of buffered NAD I used NaCL bac water and it went fine without issues. I was thinking of this thread when i recon-ed my second vial and decided to use normal BAC water, both are Hospira.
Dosing at the same dose daily, I am getting much more stinging and legit bruising now using the normal BAC water. I did a little research about it and the interweb gods tell me that it can be expected with normal BAC water with NAD.
Not sure its true or not, but i can tell you my N=1 experience. Thought I would share.
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Both MOTS and SS31 favor saline BAC, although I reconned the last batch of SS31 w/regular & it's fine.
@Eleanor My first vial of SS-31 was with sodium chloride, but on reconning my second I grabbed (by habit) my bac water. So I decided to try it and see how different it would be. Like you said, it really wasn't that different. I would say the irritation was noticeable for about 30 minutes with sodium chloride and about an hour for bac. I will admit though that reconned with twice as much bac as sodium chloride.
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@Eleanor My first vial of SS-31 was with sodium chloride, but on reconning my second I grabbed (by habit) my bac water. So I decided to try it and see how different it would be. Like you said, it really wasn't that different. I would say the irritation was noticeable for about 30 minutes with sodium chloride and about an hour for bac. I will admit though that reconned with twice as much bac as sodium chloride.
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Just out of curiosity what vender sells saline BAC?
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Just out of curiosity what vender sells saline BAC?
@charlescoyle peptidetest, it's the sodium chloride bac
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Top 20 Peptides: Reconstitution Compatibility Chart
I converted it to text so it is searchable and easier to discuss. This is a transcription of the chart—not an endorsement or independently verified protocol. If anyone has manufacturer instructions, stability studies, pharmacopeial references, or other primary sources supporting or contradicting these entries, please share them.
Peptide / Compound BAC Water BAC Saline Acetic Acid Chart’s Quick Verdict Tirzepatide 🟢 Safe 🟢 Safe 
UnnecessarySaline reduces sting Semaglutide 🟢 Safe 🟢 Safe UnnecessarySaline reduces sting Retatrutide 🟢 Safe 🟢 Safe UnnecessarySaline reduces sting BPC-157 🟢 Safe 🟢 Safe UnnecessaryHighly stable in both TB-500 (Thymosin Beta-4) 🟢 Safe 🟢 Safe UnnecessaryHighly stable in both CJC-1295 (No DAC/DAC) 🟢 Safe 🟢 Safe UnnecessarySaline reduces sting Ipamorelin 🟢 Safe 🟢 Safe UnnecessarySaline reduces sting Sermorelin 🟢 Safe 🟢 Safe UnnecessaryFully compatible Tesamorelin 🟢 Safe 🟡 Caution UnnecessarySaline slows mixing time NAD+ (Coenzyme) 🟢 Safe 🟢 Safe UnnecessarySaline heavily preferred Melanotan II 🟢 Safe 🟢 Safe UnnecessaryHighly soluble PT-141 (Bremelanotide) 🟢 Safe 🟢 Safe UnnecessaryFully compatible AOD9604 🟢 Safe 🟡 Caution 
RequiredAcid prevents clumping Frag 176-191 🟢 Safe 🟡 Caution RequiredAcid prevents clumping IGF-1 LR3/DES 
Avoid Avoid RequiredAcid prevents decay Kisspeptin-10 🟢 Safe 🟢 Safe RecommendedAcid enhances stability/solubility GHK-C (Copper Peptide) 🟢 Safe Avoid UnnecessarySalt precipitates blue MOTS-c 🟢 Safe Avoid UnnecessarySalt causes gel formation HCG 🟢 Safe 🟡 Caution UnnecessaryStandard BAC preserves longer hGH (Recombinant 191-AA) 🟢 Safe Avoid AvoidSalt/acid denatures protein Key Used in the Original Graphic
- 🟢 Safe: Presented as a compatible reconstitution solvent.
- Required/Recommended: The chart specifies diluted 0.6% acetic acid for stability or solubility.
- 🟡 Caution: The chart generally favors standard BAC water.
- Unnecessary: The chart says this solvent is not needed.
- Avoid: The chart claims the solvent may damage, denature, gel, or precipitate the compound.
Important Caveat
The word “safe” in the table only reproduces the label used by the original graphic. It should not be interpreted as proof that a preparation is sterile, stable, appropriately preserved, compatible with a particular product, or safe for human use.
Compatibility can depend on the exact formulation, salt form, concentration, pH, excipients, storage temperature, container, intended route, and manufacturer instructions. Bacteriostatic water and bacteriostatic saline are also not interchangeable in every situation.
This post is for research and discussion purposes only and is not medical, prescribing, compounding, or administration advice.
Does anyone have primary-source stability or compatibility data for these entries—especially AOD9604, Frag 176-191, IGF-1 LR3/DES, GHK-Cu, MOTS-c, and hGH?
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