Reconstitution Compatibility chart (grabbed from another group)

[pep_researcher]

@Stevepep Further

• Sterile Water, regular BAC — hypotonic, can cause tissue stress at research sites.
• Sterile saline, saline BAC — isotonic, less tissue stress at research sites.

Having said that one cannot just use saline BAC for everything to avoid research site sting, because different peps interact differently with sodium chloride.

The posted chart is interesting although it may have some errors, like Reta + Saline BAC.

[Stevepep]

@pep_researcher so is mots ok with bac saline and why don’t we use it for other peptides it’s far cheaper and if less irritating why not? Like maybe ghk-cu

[Stan Douglas]

@Snake-Pepskin
HOL' UP
You're supposed to recon IGF with 100% AA?!?!?!?
My understanding it was like... 3 drops and the rest BW.

[Eleanor]

@Stevepep said:

@pep_researcher so is mots ok with bac saline and why don’t we use it for other peptides it’s far cheaper and if less irritating why not? Like maybe ghk-cu

Go to Mot-c Reconstitution at peptide protocols (dot) app

With regard to the GHK-cu, I don't think copper will like it but I haven't checked.

[pep_researcher]

Yeah that chart isn't the source of truth. I wouldn't trust it.

[TBone]

@Stevepep @snake-pepskin I also just started researching with 100mg NAD+ (Nexaph) with 3ml BAC and no issues at all.

[Snake Pepskin]

@pep_researcher so let's correct it. What is your input/corrections?

[pep_researcher]

@Snake-Pepskin I don't have authoritative guide on which peps are compatible with saline BAC.

General thumb rule — most are compatible with regular BAC, some from those will be good with saline BAC too, some will not like sodium chloride and can gel.

I gave an example that I saw someone posting on Reddit that Reta didn't like saline BAC when they tried two Reta vials from the same batch. I asked an AI tool too and it said Reta will gel with saline BAC but tirzepatide and semaglutide will be fine with it.

Gist — that chart is NOT the ground truth. But I don't have the ground truth. Will do a chat session with multiple AI tools when I've extra time on hand and find out for popular peps.

[eurouser]

@Snake-Pepskin from my experience, AOD does not need acetic acid, been running it on my rat for months and i see no clumping and i def see results. So maybe instead of "required", the chart should say "optional" on that.

[Snake Pepskin]

I agree, mostly. AOD is one of those temperamental compounds that I would always want to have AA at hand. Kisspeptin is another one that is very ph sensitive.

[Eleanor]

Both MOTS and SS31 favor saline BAC, although I reconned the last batch of SS31 w/regular & it's fine.

[kenhnsy]

If the motivation of this topic is primarily to reduce stinging, I recommend a longer needle in a definite fatty area - butt cheeks. You will naturally diffuse it while driving to work. Ever since I went to 8mm Novafine (ceramic coated) needles for GHKCu, AOD, and NAD+, my girlfriend's big brown beaver reports very little stinging and no more welts. And this is simple to try.
Yes. Beavers. They can talk.

[JtCjEfF]

@Eleanor Interestingly, in my first vial of buffered NAD I used NaCL bac water and it went fine without issues. I was thinking of this thread when i recon-ed my second vial and decided to use normal BAC water, both are Hospira.

Dosing at the same dose daily, I am getting much more stinging and legit bruising now using the normal BAC water. I did a little research about it and the interweb gods tell me that it can be expected with normal BAC water with NAD.

Not sure its true or not, but i can tell you my N=1 experience. Thought I would share.

[Nuncles]

@Eleanor My first vial of SS-31 was with sodium chloride, but on reconning my second I grabbed (by habit) my bac water. So I decided to try it and see how different it would be. Like you said, it really wasn't that different. I would say the irritation was noticeable for about 30 minutes with sodium chloride and about an hour for bac. I will admit though that reconned with twice as much bac as sodium chloride.

[Eleanor]

@Nuncles I had a similar experience but have not noticed any sort of difference at all.

@jtcjeff NAD+ buf doesn't bother me regardless of the BAC I use, I use a lot of it. Problem solved.

[charlescoyle]

Just out of curiosity what vender sells saline BAC?

[ManicXmedic]

@charlescoyle peptidetest, it's the sodium chloride bac

[Bobbish]

Top 20 Peptides: Reconstitution Compatibility Chart

I converted it to text so it is searchable and easier to discuss. This is a transcription of the chart—not an endorsement or independently verified protocol. If anyone has manufacturer instructions, stability studies, pharmacopeial references, or other primary sources supporting or contradicting these entries, please share them.

Peptide / Compound	BAC Water	BAC Saline	Acetic Acid	Chart’s Quick Verdict
Tirzepatide	🟢 Safe	🟢 Safe	Unnecessary	Saline reduces sting
Semaglutide	🟢 Safe	🟢 Safe	Unnecessary	Saline reduces sting
Retatrutide	🟢 Safe	🟢 Safe	Unnecessary	Saline reduces sting
BPC-157	🟢 Safe	🟢 Safe	Unnecessary	Highly stable in both
TB-500 (Thymosin Beta-4)	🟢 Safe	🟢 Safe	Unnecessary	Highly stable in both
CJC-1295 (No DAC/DAC)	🟢 Safe	🟢 Safe	Unnecessary	Saline reduces sting
Ipamorelin	🟢 Safe	🟢 Safe	Unnecessary	Saline reduces sting
Sermorelin	🟢 Safe	🟢 Safe	Unnecessary	Fully compatible
Tesamorelin	🟢 Safe	🟡 Caution	Unnecessary	Saline slows mixing time
NAD+ (Coenzyme)	🟢 Safe	🟢 Safe	Unnecessary	Saline heavily preferred
Melanotan II	🟢 Safe	🟢 Safe	Unnecessary	Highly soluble
PT-141 (Bremelanotide)	🟢 Safe	🟢 Safe	Unnecessary	Fully compatible
AOD9604	🟢 Safe	🟡 Caution	Required	Acid prevents clumping
Frag 176-191	🟢 Safe	🟡 Caution	Required	Acid prevents clumping
IGF-1 LR3/DES	Avoid	Avoid	Required	Acid prevents decay
Kisspeptin-10	🟢 Safe	🟢 Safe	Recommended	Acid enhances stability/solubility
GHK-C (Copper Peptide)	🟢 Safe	Avoid	Unnecessary	Salt precipitates blue
MOTS-c	🟢 Safe	Avoid	Unnecessary	Salt causes gel formation
HCG	🟢 Safe	🟡 Caution	Unnecessary	Standard BAC preserves longer
hGH (Recombinant 191-AA)	🟢 Safe	Avoid	Avoid	Salt/acid denatures protein

Key Used in the Original Graphic

• 🟢 Safe: Presented as a compatible reconstitution solvent.
• Required/Recommended: The chart specifies diluted 0.6% acetic acid for stability or solubility.
• 🟡 Caution: The chart generally favors standard BAC water.
• Unnecessary: The chart says this solvent is not needed.
• Avoid: The chart claims the solvent may damage, denature, gel, or precipitate the compound.

Important Caveat

The word “safe” in the table only reproduces the label used by the original graphic. It should not be interpreted as proof that a preparation is sterile, stable, appropriately preserved, compatible with a particular product, or safe for human use.

Compatibility can depend on the exact formulation, salt form, concentration, pH, excipients, storage temperature, container, intended route, and manufacturer instructions. Bacteriostatic water and bacteriostatic saline are also not interchangeable in every situation.

This post is for research and discussion purposes only and is not medical, prescribing, compounding, or administration advice.

Does anyone have primary-source stability or compatibility data for these entries—especially AOD9604, Frag 176-191, IGF-1 LR3/DES, GHK-Cu, MOTS-c, and hGH?

[Stevepep]

Anyone have experience with mots-c and saline

[ManicXmedic]

@Stevepep just this past Thursday I reconned a 40mg mots-c from southern aminos with saline bac, took 5mg's and had a mild histamine reaction. Pen vial looks fine so far fwiw.