SS-31 Dosage for Researching
-
It’s worth looking at the PC posted protocol. It is pretty standard, and starts you out at lower doses before moving up to 10mg daily and eventually 50mg+ 3x/week. I doubt it would hurt you to start blasting it if you have tolerance, but you might save some money. Also, depending on your age and health, saturation dosing - which is what Pete’s doing - might not be necessary.
@ResearchCat I agree. The 1gram protocol here is pretty solid, I THINK @peptidepete did it, and upped it on the 2nd protocol.
I did the 1gram which titrates up and ends with a high dose of ss-31.
If you already did it then i think for a 2nd round makes sense.
-
I am only at the 4 mg a day dose right now after ~6 weeks. I am 53 and will start low and work up to 50mg a day for 4-8 weeks somewhere I think. I am waiting for the peps which will take some time to get and I want to hear how @peptidepete does on his current 60mg run. I really would love to see some eyesight improvements from it. My eyesight is bad and getting worse.
-
About 6 weeks ago I restarted my SS-31 research at 60mg per day, every day. Following up on my previously posted multi-month course of 40mg per day. I feel great, no negatives. Some subjective improvements. Will conclude research at 8 weeks daily use (two weeks from now). I am very well optimized and use this to ameliorate any possible negs from compounds such as O-304 and/or occasional AAS oral/oil.
Mitochondrial related bloodwork is pristine, as 65 years old, so N=1 is thrilled.
There is zero evidence that microdosing gives any results. The one human trial at 4mg showed zero efficacy. Zero. Only 40mg daily showed results.
Go big or don't bother is my opinion of this material.
Know what you are doing and why you are doing it.
There is very thought-provoking discussion "out there" in regards to the damage leaky membranes (cardiolipin) cause. ROS, free-radicals, etc. Very nasty. What are the downstream affects of that, not fcking good that's what.
SS-31 will be something I run multiple times per year. The benefits are NOT one-and-done. Pull on that string. Gotta keep working on that particular pathway.
Research is your friend.
@PeptidePete - ...or anyone else who has done the 1 gram protocol at listed. When you are doing your high doses of 50mg, what concentration are you reconstituting to? Thanks!
-
@PeptidePete - ...or anyone else who has done the 1 gram protocol at listed. When you are doing your high doses of 50mg, what concentration are you reconstituting to? Thanks!
@Blueeyedme i think that all depends on your supply. If you get a ss-31 vial of 50mg you add 1 ml and pin the hole bottle
-
Agree with diegoc. I don’t have issues pinning SS-31, so it’s a matter of how much you can or are willing to stuff into a cart, and how much you want to inject. I think my last run of 10mg/day x 30 days was 40-ish units per day but that was mainly because i only had 10mg vials. Speaking of, I need to order more from Nexaph. along with some other stuff.
-
Agree with diegoc. I don’t have issues pinning SS-31, so it’s a matter of how much you can or are willing to stuff into a cart, and how much you want to inject. I think my last run of 10mg/day x 30 days was 40-ish units per day but that was mainly because i only had 10mg vials. Speaking of, I need to order more from Nexaph. along with some other stuff.
@ResearchCat i agree. If your doing a high dose ss-31 protocol buy a kit or kits and calculate the entire protocol.
Your pocket book will tell you how much you can dose and for how long
-
@Blueeyedme i think that all depends on your supply. If you get a ss-31 vial of 50mg you add 1 ml and pin the hole bottle
@diegoc - Cute...I've got plenty on hand in 100 & 150mg vials. What I am asking is if anyone knows the solubility limit for SS-31? If not, I'll figure it out. Thanks!
-
Anyone doing Urolithin A along with any mito stack? There’s a ton of human data on Urolithin A for mito health and even muscle and cognitive health. I’m doing it along with ss-31 10mg daily before I start Mots. Human data with Urolithin A says to run it at least 4 months for muscle benefit.
-
@diegoc - Cute...I've got plenty on hand in 100 & 150mg vials. What I am asking is if anyone knows the solubility limit for SS-31? If not, I'll figure it out. Thanks!
@Blueeyedme on a high dose vial like that i would mix 1ml bac and do 2 shots of 50mg, the 150mg vial you can do 1.5ml bac
-
@Blueeyedme on a high dose vial like that i would mix 1ml bac and do 2 shots of 50mg, the 150mg vial you can do 1.5ml bac
@diegoc - that makes sense. Keeps the volume at a comfortable 50 units with a 100mg/mL concentration. Hopefully at that concentration it remains stable and fully dissolved.
-
About 6 weeks ago I restarted my SS-31 research at 60mg per day, every day. Following up on my previously posted multi-month course of 40mg per day. I feel great, no negatives. Some subjective improvements. Will conclude research at 8 weeks daily use (two weeks from now). I am very well optimized and use this to ameliorate any possible negs from compounds such as O-304 and/or occasional AAS oral/oil.
Mitochondrial related bloodwork is pristine, as 65 years old, so N=1 is thrilled.
There is zero evidence that microdosing gives any results. The one human trial at 4mg showed zero efficacy. Zero. Only 40mg daily showed results.
Go big or don't bother is my opinion of this material.
Know what you are doing and why you are doing it.
There is very thought-provoking discussion "out there" in regards to the damage leaky membranes (cardiolipin) cause. ROS, free-radicals, etc. Very nasty. What are the downstream affects of that, not fcking good that's what.
SS-31 will be something I run multiple times per year. The benefits are NOT one-and-done. Pull on that string. Gotta keep working on that particular pathway.
Research is your friend.
@PeptidePete```
Thanks Pete, much appreciated on the update. Definitely multiple courses here per year. I turn mine up each time as well. It’s been a game changer. -
Update- just finished the course last week, crazy strong urine stream (I'm 65 yr old), unexpected effect but awesome...lol
May be a synergistic combination with Klotho, so I'd be super curious to hear other data points here.This material gives most people a bump and itch and redness for a few hours or less. I suggest doing oral antihistamine until you acclimate.
QSC/Tracy (love them) has it for .70/mg
In regards to reconstitution I worked up to tolerating 1ml BAC to 50-60mg vial, but I also tried 2ml split between two pins. Do what works for you.
In regards to doses under 40mg/day, there is no evidence for efficacy that I have seen, if someone has it, please post.
I am a bit surprised, but heartened, to read the enthusiasm and success reports! Do you own deep research and do what is best for you, for sure.
-
Update- just finished the course last week, crazy strong urine stream (I'm 65 yr old), unexpected effect but awesome...lol
May be a synergistic combination with Klotho, so I'd be super curious to hear other data points here.This material gives most people a bump and itch and redness for a few hours or less. I suggest doing oral antihistamine until you acclimate.
QSC/Tracy (love them) has it for .70/mg
In regards to reconstitution I worked up to tolerating 1ml BAC to 50-60mg vial, but I also tried 2ml split between two pins. Do what works for you.
In regards to doses under 40mg/day, there is no evidence for efficacy that I have seen, if someone has it, please post.
I am a bit surprised, but heartened, to read the enthusiasm and success reports! Do you own deep research and do what is best for you, for sure.
@PeptidePete does Tracy have any recent COA’s for SS31 - 50? The last one I saw was 2025.
-
Growth Garage has SS-31 50mg kits for $130 in their current GB. Do you think there is a chance they are good? Or it's too cheap to be not a fake or bad?
@vpeps do you trust that the person selling this out of there garage is actually batch testing what is being sent? Do you trust that if the buy doesnt go through for some reason you will get a refund?
A vendor is good until they aren't.
A group buy organizer has a clock. Eventually it runs out.
$90 is the price you start to get to when you are buying 200+ kits at a time so no i don't think 130 is crazy.
-
Update- just finished the course last week, crazy strong urine stream (I'm 65 yr old), unexpected effect but awesome...lol
May be a synergistic combination with Klotho, so I'd be super curious to hear other data points here.This material gives most people a bump and itch and redness for a few hours or less. I suggest doing oral antihistamine until you acclimate.
QSC/Tracy (love them) has it for .70/mg
In regards to reconstitution I worked up to tolerating 1ml BAC to 50-60mg vial, but I also tried 2ml split between two pins. Do what works for you.
In regards to doses under 40mg/day, there is no evidence for efficacy that I have seen, if someone has it, please post.
I am a bit surprised, but heartened, to read the enthusiasm and success reports! Do you own deep research and do what is best for you, for sure.
I wont argue your efficacy under 40mg but i definitely feel effects at 20mg. Thats is actual first hand evidence for you even if not a study. Of course i dont have a mitochondrial disease so it make sense that lower doses still work for me.
I disagree with discouraging lower effective doses than 40mg for this pep. By doing so it unnecessarily discourages lower income users from this product when many could still receive benefits from even one 50mg kit.
I found that titrating up to the detox point where you feel crap, holding til it passes then moving up rapidly works. My first benefits were had starting around 10-12mg per day. At 15-17 i hit another detox.
I have a working theory that that slow sluggish period that happens is actually a mitochondial detox perhaps removing senescent cells. Which would remove the need for urolothin a or 5-amino
-
@vpeps do you trust that the person selling this out of there garage is actually batch testing what is being sent? Do you trust that if the buy doesnt go through for some reason you will get a refund?
A vendor is good until they aren't.
A group buy organizer has a clock. Eventually it runs out.
$90 is the price you start to get to when you are buying 200+ kits at a time so no i don't think 130 is crazy.
@Randy I treat it like Vegas you win and lose on group buys. I play the game but know it’s an all or nothing proposition. If you do enough of them you are going to get a zero peptide, if you test, or not get product at all. There is pretty much no recourse at that point. So you just play your next hand. I will never understand how people just accept the COA from the buy. Sometimes they post it weeks before they receive it the actual products, just nuts
-
Update- just finished the course last week, crazy strong urine stream (I'm 65 yr old), unexpected effect but awesome...lol
May be a synergistic combination with Klotho, so I'd be super curious to hear other data points here.This material gives most people a bump and itch and redness for a few hours or less. I suggest doing oral antihistamine until you acclimate.
QSC/Tracy (love them) has it for .70/mg
In regards to reconstitution I worked up to tolerating 1ml BAC to 50-60mg vial, but I also tried 2ml split between two pins. Do what works for you.
In regards to doses under 40mg/day, there is no evidence for efficacy that I have seen, if someone has it, please post.
I am a bit surprised, but heartened, to read the enthusiasm and success reports! Do you own deep research and do what is best for you, for sure.
@PeptidePete
Thanks for posting the update!
BTW, SS-31 works so well for me at 5mg that I cannot combine it with caffeine! My mito are in sad shape, though; that probably has a lot to do with it...big difference when I'm off of it. -
@peptidepete Your post is great. Appreciate the way it's laid out.
-
@MacLeeezy said in SS-31 Dosage for Researching:
Any thoughts on the recent study: Gudiksen et al., Free Radical Biology and Medicine (2026) that talks about potentially running SS31 and Mots-c together instead of Ss31 first and then Motsc?
Just curious how your mice prefer to run both? Together or staggered?
I have only ever considered running both at the same time. Complimentary in every way. No such thing as "repair" then "grow" in my point of view, silly concept.
My stack: SS-31/NAD+/MOTS/5-AMINO to start, titrated up in frequency/dose, then when I completed and discontinued the SS-31 50mg per day for 60 days, added O-304/SLU/BAM-15 to the others. LOVE IT. Keep in mind, I am very well optimized in general, so working on the top of the pyramid. Not the stack for everybody depending upon individual sensitivities.
Freebie- if you are serious about optimizing mitochondria, you darn well better be incorporating the very best EPA/DHA/DPA sources, specifically fish eggs, preferably sturgeon (suggest the reader research it as I did). The fatty acids are taken up into the all your cell membranes over time. It's freaking crazy stuff, search it and learn it. No one ever talks about this...except me. A "superpower" addition to a serious and effective mitochondrial stack. You're welcome
@PeptidePete love the idea of adding caviar to my diet. How much do you take daily? A tablespoon? Or more? I’m almost done with the protocol for SS – 31. Thank you!
Hello! It looks like you're interested in this conversation, but you don't have an account yet.
Getting fed up of having to scroll through the same posts each visit? When you register for an account, you'll always come back to exactly where you were before, and choose to be notified of new replies (either via email, or push notification). You'll also be able to save bookmarks and upvote posts to show your appreciation to other community members.
With your input, this post could be even better 💗
Register Login