SS-31 (Elamipretide) — Research Dosing Protocol

[MacLeeezy]

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[MacLeeezy]

Hey good Afternoon. I have a 10pack of 50mg SS31 on the way to research with. This is about 14 vials short of being able to complete this protocol.

I am looking to do a miniature version of this and would appreciate any feedback from the community on if you think this will work well given my limited supplies or if I’m titrating up too quickly:

Weeks 1-3: 4mg Daily
Weeks 4-6: 8mg Daily
Weeks 7-9: 25mg 3 times per week

Thanks!

[diegoc]

@MacLeeezy im doing this protocol and i bought the 1gr required.

If you dont have enough probably cut on the 25mg and lower that dosez

[iMemphis18]

My rat just got ss-31 in today and after reading this, I think

Weeks 1-3: 4mg Daily
Weeks 4-6: 8mg Daily

should work yes?

@macleeezy @diegoc since you guys are both using this for your rats also, did you guys notice anything when doign 4mg right off the bat?

just to confirm:

Using the peptide calculator,

Desired dose is 4MG,
Vial size is 10MG
2ML bac water

I would need to give my Rat 80 units daily?

[PeptideEd]

@imemphis I've done 4mg (just finished that part and moved to 10mg in the last few days) - was a pretty easy adjustment. That said, and with the caveat that the following comment is a matter of taste, I'd go with 1 mL to a 10mg vial and do 40 units. From where I sit, for me, 80 units is a lot in an injection. When I did 4mg I did specifically 10mg/mL concentration and thus 40 units for 4mg. To be clear, its a matter of taste, there's nothing wrong with your plan if 80 unit shots are comfortable for you

[iMemphis18]

Thanks for the heads up @peptideed !

[PeptideEd]

@imemphis oh... and when I transitioned to 10mg a day recently, I switched to a 20mg/mL concentration (50 units to get 10mg). Injection went smoothly.

[MacLeeezy]

@iMemphis I’m just planning for the future. I have a kit of SS-31 but haven’t started it yet. Planning on running fox04-dri first but need to finish up a couple other thing first

[ResearchCat]

I just finished my SS-31 run. I decided to do phases 1-2 and skip the burst phase mainly due to costs. I did a concentration of 10mg in 40 units (~24mg/ml). I occasional mild ISR but no worse than from 12 units of glow. I found using the gansulin pen made it a breeze.

Now running MOTS-C and planning to add NAD+.

[MyB]

@researchcat Dr Sawicki dropped a short on YouTube about how antioxidants block Mot-C from entering the cell. So you’ll probably want to research Mot and NAD on different runs.
https://youtube.com/shorts/W2a9qZ7vP1k?si=3-iRJFN2so7hVyLx

[ResearchCat]

@MyB that’s interesting. My inclination was to do them all separately, but it seems a lot of people (YouTubers) are suggesting you blast everything all together. Appreciate the tip.

Edit: Oh, I see, so not saying you can’t research them together, but that you shouldn’t research them at the same time of day.

[MyB]

@researchcat i made a post under the mot-c dosing topic. https://community.peptidecritic.com/post/2819
Based on half life’s, dosing Mot-C then dose NAD+ 8 hrs later would get full benefits of both without overlap.

[MacLeeezy]

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[Katya]

@diegoc newbie here.

How lengthy is the Fox04 protocol?
Would a fasting protocol work similarly to Fox04?

TIA

[diegoc]

I would think fasting works on Autophogy and reset, and Fox04 clears scenesant cells.

Many different protocols for length of time

[PeptidePete]

said in SS-31 (Elamipretide) — Research Dosing Protocol:

@Randy said in SS-31 (Elamipretide) — Research Dosing Protocol:

@PeptidePete let us know how it goes. I just finished it a few days ago. My only real complaint is the itchy ISR i got as the dose went up.

Currently finishing up some 40mg vials which I was reconstituting with <1ml and it was too uncomfortable at that concentration in one pin in one spot. Switched to doubling the dilution, two 1ml pins in adjacent sites. Everything hunky-dory now.

Also was pinning in the AM, now switched to PM as my pin load is less at night. Less chance of cross-talk issues, and perhaps its magic works better while the body is doing other clean up. Who knows.

Finished the 50mg daily blast over 2+ months. Can't say I feel any different but then again I felt pretty damn good to start with and was 95% optimized. Other than mild ISR no sides. Should have some flipping good mitochondria now...lol. PS- as previously noted, I would suggest titration over some period of time and not just starting up 50mg from the get go. To each his own.

Still running MOTS/NAD+ because, well, I love the NAD and like the MOTS.

Bonus tip for ultimate mito health maintenence- fish eggs (roe), and if your finances allow, specifically sturgeon roe. Bought in 500g or 1kg tins, it is not that much more of an investment compared to some peptides. Your research on incorporation into cellular membranes will blow your mind. Explains and dovetails nicely with man's spread across the earth via coastal/ocean routes.

[justalittleprick]

I've seen SS-31 recommendations ranging from 2-4mg daily up to 40mg daily. It sounds like the 40mg comes from a trial for people with Barth syndrome, which is really rare and an extreme case of mitochondrial disfuntion.

For those of you researching SS-31 in the 2-4mg daily range along with or as a precursor to MOTS-C, were you happy with the effects or do you think higher doses are required?

[ResearchCat]

@PeptidePete Yeah, I don’t know that you are necessarily supposed to feel something from the SS-31 if you don’t have issues, but it should repair things that aren’t working and lay the groundwork for the MOTS-C and NAD+. I started MOTS-C a couple weeks ago and feel some more energy and no side effects. Added NAD+ this week and that has a kick that is almost scary(started at 50mg).

It is tough to tell the difference day over day without journaling, especially if you are measuring how good you feel. From pictures, measures, records, and third party opinion, I am much improved from last year, when i started out. Feel great, less pain, lift heavier, look better, people say I look great, scans say I have less fat. I need to work on inflammation, cardiac indicators, and improving muscle mass, but it is all working. I look and feel better than when i was 20 years younger.

Further along those lines, if you believe some of the research on NAD+ and cardiac health, then running the SS/MOTS/NAD protocol could have meaningful benefits for heart and overall health that might not be easy to measure without frequent, expensive testing. I guess if I live to be 100 and still have mobility and a few marbles, it worked, and if I don’t, well, I’ve spent money on dumber things.

[ResearchCat]

@justalittleprick I think of SS-31 as more laying the groundwork than building the structure. You might try one of the protocols that used SS-31, MOTS-C, and NAD+ for more immediate effect. I didn’t necessarily feel any different on SS-31, the same as I didn’t feel anything from epitalon, but that doesn’t mean it isn’t working. I am also researching other things, so it’s hard to know with certainty what is doing what if you are getting the desired results.

My opinion is that a lower dose is probably fine for baseline repair, but that depends on your age, fitness, etc. If you’re 20-something, you probably don’t need any of this stuff. If you’re 60-something, you’ll probably be amazed what the mitochondrial reset will do for your energy levels.

[diegoc]

I agree. Im in the 10mg daily phase of the ss-31 protocol.

I dont feel anything but like you said, its repairing the mitochondria.

I do know when i add the Mots-c i will feel the difference.